Carbon Nanohorns as a Scaffold for the Construction of Disposable Electrochemical Immunosensing Platforms. Application to the Determination of Fibrinogen in Human Plasma and Urine

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dc.contributor.authorOjeda Fernández, Irene
dc.contributor.authorGarcinuño, Belit
dc.contributor.authorMoreno Guzmán, María
dc.contributor.authorGonzález Cortés, Araceli
dc.contributor.authorYudasaka, Masako
dc.contributor.authorIijima, Sumio
dc.contributor.authorLanga, Fernando
dc.contributor.authorYáñez-Sedeño Orive, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-08T14:15:56Z
dc.date.available2024-01-08T14:15:56Z
dc.date.issued2014
dc.description.abstractWe describe in this work a novel electrochemical immunosensor design making use of carbon nanohorns (CNHs) as a scaffold for the preparation of disposable immunosensing platforms for the determination of fibrinogen (Fib). The approach involved the immobilization of Fib onto activated CNHs deposited on screen-printed carbon electrodes (SPCEs) and the implementation of an indirect competitive assay using anti-Fib labeled with horseradish peroxidase (HRP) and hydroquinone (HQ) as the redox mediator. Both CNHs and the Fib-CNHs covalent assembly were characterized by microscopic and electrochemical techniques. The different variables affecting the analytical performance of the amperometric immunosensing strategy were optimized. The calibration plot for Fib allowed a range of linearity between 0.1 and 100 μg/mL (r 2 = 0.994) and a detection limit of 58 ng/ mL to be achieved. The Fib-CNHs/SPCEs exhibited an excellent storage stability of at least 42 days. The developed immunosensor provides, in general, an analytical performance better than that reported for other Fib immunosensors and commercial ELISA kits. This simple and relatively low cost immunosensor configuration permitted the sensitive and selective determination of Fib in human plasma and urine
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y ́Competitividad (España)
dc.description.statuspub
dc.identifier.citationAnal. Chem. 2014, 86, 15, 7749–7756 Publication Date:July 7, 2014 https://doi.org/10.1021/ac501681n
dc.identifier.doi10.1021/ac501681n
dc.identifier.issn0003-2700
dc.identifier.officialurlhttps://doi.org/10.1021/ac501681n
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91826
dc.journal.titleAnalytical Chemistry
dc.language.isoeng
dc.page.final7756
dc.page.initial7749
dc.publisherACS
dc.relation.projectID(project CTQ2012-35041)
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.keywordEngineering controlled terms
dc.subject.keywordBody fluids
dc.subject.keywordImmunosensors
dc.subject.keywordPlasma (human)
dc.subject.keywordScaffolds
dc.subject.keywordAmperometric immunosensing
dc.subject.keywordElectrochemical immunosensing
dc.subject.keywordElectrochemical immunosensors
dc.subject.keywordElectrochemical techniques
dc.subject.keywordHorseradish peroxidase
dc.subject.keywordIndirect competitive assays
dc.subject.keywordScreen printed carbon electrode (SPCEs)
dc.subject.keywordSelective determination
dc.subject.keywordNanohorns
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleCarbon Nanohorns as a Scaffold for the Construction of Disposable Electrochemical Immunosensing Platforms. Application to the Determination of Fibrinogen in Human Plasma and Urine
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number86
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscoveryb4631aae-bbc4-470a-922b-73f321e2515a
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