Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain

Simple item page
dc.contributor.authorSantín, Mónica
dc.contributor.authorCalero Bernal, Rafael
dc.contributor.authorCarmena, David
dc.contributor.authorMateo Barrientos, Marta
dc.contributor.authorBalseiro, Ana
dc.contributor.authorBarral, Marta
dc.contributor.authorLima Barbero, José Francisco
dc.contributor.authorHabela, Miguel Ángel
dc.date.accessioned2024-02-05T08:18:33Z
dc.date.available2024-02-05T08:18:33Z
dc.date.issued2017-12-22
dc.description.abstractMicrosporidia comprises a diverse group of obligate intracellular parasites that infect a broad range of invertebrates and vertebrates. Among Microsporidia, Enterocytozoon bieneusi is the most frequently detected species in humans and animals worldwide bringing into question the possible role of animal reservoirs in the epidemiology of this pathogen. Although E. bieneusi is an emerging zoonotic pathogen able to infect many domestic and wild mammals that could act as reservoir of infection for humans and other animals, only few studies have documented its occurrence in wild carnivores. To determine the occurrence of E. bieneusi in wild carnivores, we examined 190 wild carnivores collected from different locations in Spain. Twenty-five fecal samples (13.2%) from three host species (European badger, beech marten, and red fox) were E. bieneusi-positive by PCR. Nucleotide sequence analysis of the ITS region revealed a high degree of genetic diversity with a total of eight distinct genotypes including four known (PtEbIX, S5, S9, and WildBoar3) and four novel (EbCar1-EbCar4) genotypes identified. Phylogenetic analysis showed that the four novel genotypes (EbCar1-EbCar4), S5, S9, and WildBoar3 clustered within the previously designated zoonotic Group 1. Our results demonstrate that human-pathogenic genotypes are present in wild carnivores, corroborating their potential role as a source of human infection and environmental contamination.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationSantín, M., Calero-Bernal, R., Carmena, D., Mateo, M., Balseiro, A., Barral, M., Lima Barbero, J. F., & Habela, M. Á. (2018). Molecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain. The Journal of eukaryotic microbiology, 65(4), 468–474. https://doi.org/10.1111/jeu.12492
dc.identifier.doi10.1111/jeu.12492
dc.identifier.essn1550-7408
dc.identifier.issn1066-5234
dc.identifier.officialurlhttps://onlinelibrary.wiley.com/doi/10.1111/jeu.12492
dc.identifier.pmid29230898
dc.identifier.urihttps://hdl.handle.net/20.500.14352/98623
dc.issue.number4
dc.journal.titleJournal of Eukaryotic Microbiology
dc.language.isoeng
dc.page.final474
dc.page.initial468
dc.publisherWiley
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordPCR
dc.subject.keywordGenotypes
dc.subject.keywordMolecular epidemiology
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleMolecular Characterization of Enterocytozoon bieneusi in Wild Carnivores in Spain
dc.typejournal article
dc.type.hasVersionP
dc.volume.number65
dspace.entity.typePublication
relation.isAuthorOfPublicationddeaf49e-38b4-40ed-98fa-0031ae42f6eb
relation.isAuthorOfPublication922775f7-7c98-4ef5-a412-d701cbdc0f91
relation.isAuthorOfPublication.latestForDiscoveryddeaf49e-38b4-40ed-98fa-0031ae42f6eb
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Santín_et_al-2018-Journal_of_Eukaryotic_Microbiology.pdf
Size:
435.98 KB
Format:
Adobe Portable Document Format
Download
Collections
Artículos