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A bump-hole strategy for increased stringency of cell-specific metabolic labeling of RNA

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dc.contributor.authorNguyen, Kim
dc.contributor.authorKubota, Miles
dc.contributor.authorDel Arco, Jon
dc.contributor.authorFeng, Chao
dc.contributor.authorSingha, Monika
dc.contributor.authorBeasley, Samantha
dc.contributor.authorSakr, Jasmine
dc.contributor.authorGandhi, Sunil P.
dc.contributor.authorBlurton-Jones, Matthew
dc.contributor.authorFernández Lucas, Jesus
dc.contributor.authorSpitale, Robert C.
dc.date.accessioned2024-10-24T18:12:14Z
dc.date.available2024-10-24T18:12:14Z
dc.date.issued2020-11
dc.descriptionRNA research in the Spitale lab is supported by startup funds from the University of California, Irvine, the NIH Director’s New Innovator Award (1DP2GM119164 RCS). S.B. is supported by an NIH training grant 5T32CA009054-40.
dc.description.abstractProfiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipUniversity of California (Berkeley)
dc.description.statuspub
dc.identifier.citationNguyen K, Kubota M, Del Arco J, Feng C, Singha M, Beasley S, Sakr J, Gandhi SP, Blurton-Jones M, Fernández Lucas J, Spitale RC. A Bump-Hole Strategy for Increased Stringency of Cell-Specific Metabolic Labeling of RNA. ACS Chem Biol 2020;15:3099–105. https://doi.org/10.1021/acschembio.0c00755.
dc.identifier.doi10.1021/acschembio.0c00755
dc.identifier.essn1554-8937
dc.identifier.issn1554-8929
dc.identifier.officialurlhttps://doi.org/10.1021/acschembio.0c00755
dc.identifier.relatedurlhttps://pubs.acs.org/doi/10.1021/acschembio.0c00755
dc.identifier.urihttps://hdl.handle.net/20.500.14352/109478
dc.issue.number12
dc.journal.titleACS Chemical Biology
dc.language.isoeng
dc.page.final3105
dc.page.initial3099
dc.publisherAmerican Chemical Society
dc.rights.accessRightsopen access
dc.subject.cdu577.15
dc.subject.cdu577.2
dc.subject.cdu575
dc.subject.keywordGenetics
dc.subject.keywordImaging probes
dc.subject.keywordLabeling
dc.subject.keywordPeptides and proteins
dc.subject.keywordUracil
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmBiología molecular (Biología)
dc.subject.ucmGenética
dc.subject.unesco2403 Bioquímica
dc.subject.unesco2415 Biología Molecular
dc.subject.unesco2302.09 Enzimología
dc.subject.unesco2302.04 Genética Bioquímica
dc.titleA bump-hole strategy for increased stringency of cell-specific metabolic labeling of RNA
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number15
dspace.entity.typePublication
relation.isAuthorOfPublicationf99cf5b4-0f0d-424c-afd9-77bdedffd366
relation.isAuthorOfPublication.latestForDiscoveryf99cf5b4-0f0d-424c-afd9-77bdedffd366

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