Optimized Protocol for Characterization of Mouse Gut Innate Lymphoid Cells

Simple item page
dc.contributor.authorValle Noguera, Ana
dc.contributor.authorGómez Sánchez, María José
dc.contributor.authorGirard Madoux, Mathilde J. H.
dc.contributor.authorCruz Adalia, Aranzazu
dc.date.accessioned2024-01-10T07:45:42Z
dc.date.available2024-01-10T07:45:42Z
dc.date.issued2020-11-30
dc.description.abstractSince their discovery, innate lymphoid cells (ILCs) have gradually been gaining greater relevance in the field of immunology due to their multiple functions in the innate immune response. They can mainly be found in mucosal and barrier organs like skin, gut, and lungs, and have been classified into five main types (NKs, ILC1s, ILC2s, ILC3s, and Lti cells) according to their function and development. They all play major roles in functions such as tissue homeostasis, early pathogen defense, regulation of inflammation, or tissue remodeling. ILCs are mostly tissue-resident cells tightly bound to the tissue structure, a fact that requires long and complex protocols that do not always provide sufficient yield for analysis. This suggests the need for optimized approaches aimed at ensuring that enriched and viable ILC samples are obtained, in order to furnish quality results. Herein a detailed protocol is established for obtaining a single-cell suspension highly enriched in lymphoid cells from mouse gut in order to identify the different subsets of ILCs by means of flow cytometry. The cell marker panel and flow cytometry gating strategies for identification and quantification of all the different ILC populations are provided for simultaneous analysis. Moreover, the protocol described includes a procedure for studying the different cytokines produced by ILC3s involved in maintaining the integrity of the gut barrier and defending against extracellular pathogens. As a result, herein an efficient method is presented for studying mouse ILCs within the lamina propria of the small intestine and colon; this can constitute a useful tool for future investigations in the field.
dc.description.departmentDepto. de Inmunología, Oftalmología y ORL
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación e Universidades
dc.description.sponsorshipAgenda Estatal de Investigación
dc.description.sponsorshipFondo Europeo de Desarrollo Regional
dc.description.statuspub
dc.identifier.citationValle-Noguera A, Gómez-Sánchez MJ, Girard-Madoux MJH, Cruz-Adalia A. Optimized Protocol for Characterization of Mouse Gut Innate Lymphoid Cells. Front Immunol. 2020 Nov 30;11:563414. doi: 10.3389/fimmu.2020.563414. PMID: 33329525; PMCID: PMC7735015
dc.identifier.doi10.3389/fimmu.2020.563414
dc.identifier.issn1664-3224
dc.identifier.relatedurlhttps://www.frontiersin.org/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92149
dc.journal.titleFrontiers in Immunology
dc.language.isoeng
dc.publisherFrontiers Media
dc.relation.projectIDRTI2018- 093647-B-I00
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu612.017
dc.subject.keywordinnate lymphoid cells
dc.subject.keywordflow cytometry
dc.subject.keywordsmall intestine
dc.subject.keywordcolon
dc.subject.keywordlamina propria cells
dc.subject.keywordinnate lymphoid cell type3
dc.subject.keywordgut immunology
dc.subject.keywordILC3 cytokines
dc.subject.ucmInmunología
dc.subject.unesco2412.99 Otras
dc.titleOptimized Protocol for Characterization of Mouse Gut Innate Lymphoid Cells
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number11
dspace.entity.typePublication
relation.isAuthorOfPublicationd7ccfedb-f3d6-43c6-b3c7-1196d375be31
relation.isAuthorOfPublicationae965912-b825-4a38-98db-737d69d3759a
relation.isAuthorOfPublication.latestForDiscoveryd7ccfedb-f3d6-43c6-b3c7-1196d375be31
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fimmu-11-563414.pdf
Size:
3.16 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos