Effect of a Combined Treatment With Growth Hormone and Melatonin in the Cardiological Aging on Male SAMP8 Mice
Loading...
Full text at PDC
Publication date
2011
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press
Citations
Exportar
Citation
Katherine Forman, Elena Vara, Cruz García, Roman Kireev, Sara Cuesta, Germaine Escames, J. A. F. Tresguerres, Effect of a Combined Treatment With Growth Hormone and Melatonin in the Cardiological Aging on Male SAMP8 Mice, The Journals of Gerontology: Series A, Volume 66A, Issue 8, August 2011, Pages 823–834, https://doi.org/10.1093/gerona/glr083
Abstract
The effect of a chronic combined treatment with growth hormone (GH) plus melatonin (Mel) on different age-related processes in cytosolic and nuclear fractions of hearts from SAMP8 mice (2 and 10 months) has been investigated. The parameters studied have been messenger RNA expressions of IL-1, IL-10, NFkBp50, NFkBp52, TNFα, eNOS, iNOS, HO-1, HO-2, BAD, BAX, and Bcl2 and protein expressions of iNOS, eNOS, TNFα, IL-1, IL-10, NFkBp50, NFKbp52, and caspase activity (3 and 9). Our results supported the existence of a proapoptotic and oxidative status together with inflammatory processes in the heart of old mice, with increases of inflammatory cytokines, caspase activity, HO-1, BAX, NFkBp50, and NFkBp52 and decreases of eNOS and Bcl2. Also, we were able to observe the translocation of NFkB to nuclei. The combined treatment was able to partially reduce the incidence of these deleterious changes, showing differences with the separated treatments with GH and Mel as were investigated in previous articles from our group.