Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production

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dc.contributor.authorGuevara Acosta, Flor Govinda
dc.contributor.authorOlortegui Flores, Yamileth
dc.contributor.authorFernández de las Heras, Laura
dc.contributor.authorPerera González, Julián
dc.contributor.authorNavarro Llorens, Juana María
dc.date.accessioned2023-06-17T13:34:43Z
dc.date.available2023-06-17T13:34:43Z
dc.date.issued2019-07-26
dc.description.abstractRhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of the steroid catabolism inside the strain. A R. ruber quadruple mutant was constructed in order to avoid the breakage of the steroid nucleus. At the same time, an inducible expression vector for this strain was developed. The 17-ketoreductase gene from the fungus Cochliobolus lunatus was cloned and overexpressed in this vector. The engineered strain was able to produce testosterone from 4-androstene-3,17-dione using glucose for cofactor regeneration with a molar conversion of 61%. It is important to note that 91% of the testosterone was secreted outside the cell after 3 days of cell biotransformation. The results support the idea that Rhodococcus ruber Chol-4 can be metabolically engineered and can be used for the production of steroid intermediates.en
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.facultyFac. de Ciencias Biológicas
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/57927
dc.identifier.citationGuevara G, Olortegui Flores Y, Fernández de Las Heras L, Perera J, Navarro Llorens JM. Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production. PLoS One. 2019 Jul 26;14(7):e0220492. doi: 10.1371/journal.pone.0220492. PMID: 31348804; PMCID: PMC6660089.
dc.identifier.doihttps:// doi.org/10.1371/journal.pone.0220492
dc.identifier.issn1932-6203
dc.identifier.officialurlhttps:// doi.org/10.1371/journal.pone.0220492
dc.identifier.relatedurlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0220492
dc.identifier.urihttps://hdl.handle.net/20.500.14352/13790
dc.issue.number0220492
dc.journal.titlePlos One
dc.language.isoeng
dc.page.final16
dc.page.initial1
dc.page.total16
dc.publisherPublic Library of Science
dc.relation.projectID(RTC2014-2249-1)
dc.relation.projectIDinfo:eu-repo/grantAgreement/ Ministerio de Ciencia e Innovación//RTC2014-2249-1
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu577.175.62
dc.subject.cdu579.222.6
dc.subject.keywordRhodococcus Chol-4
dc.subject.keywordTestosterone
dc.subject.keywordMetabolic engineering
dc.subject.keywordSteroid
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmMicrobiología (Biología)
dc.subject.unesco2302 Bioquímica
dc.subject.unesco2414 Microbiología
dc.titleMetabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone productionen
dc.title.alternativeIngeniería metabólica de Rhodococcus ruber Chol-4: Una factoría celular para la producción de testosteronaes
dc.typejournal article
dc.volume.number14
dspace.entity.typePublication
relation.isAuthorOfPublication41a4d8da-9d33-4a5e-8dab-373eec22fcbe
relation.isAuthorOfPublication5a9a01b6-fb5f-422a-b3d0-74ad581e2cbd
relation.isAuthorOfPublicationd0b7aecd-194b-4ea4-b073-f5b1ec49a255
relation.isAuthorOfPublication.latestForDiscovery5a9a01b6-fb5f-422a-b3d0-74ad581e2cbd
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