Grape antioxidant dietary fibre prevents mitochondrial apoptotic pathways by enhancing Bcl-2 and Bcl-xL expression and minimising oxidative stress in rat distal colonic mucosa.

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dc.contributor.authorLópez-Oliva Muñoz, María Elvira
dc.contributor.authorPozuelo de Felipe, María José
dc.contributor.authorRotger, Rafael
dc.contributor.authorMuñoz Martínez, Emilia
dc.contributor.authorGoñi, Isabel
dc.date.accessioned2023-06-20T03:59:03Z
dc.date.available2023-06-20T03:59:03Z
dc.date.issued2012-03-19
dc.description.abstractGrape antioxidant dietary fibre (GADF) is a grape product rich in dietary fibre and natural antioxidants. We reported previously that GADF intake reduced apoptosis and induced a pro-reducing shift in the glutathione (GSH) redox status of the rat proximal colonic mucosa. The aim of the study was to elucidate the molecular mechanisms responsible for the anti-apoptotic effect of GADF and their association with the oxidative environment of the distal colonic mucosa. The ability of GADF to modify colonic crypt cell proliferation was also investigated. Male Wistar rats (n 20) were fed with diets containing either cellulose (control group) or GADF (GADF group) as fibre for 4 weeks. GADF did not modify cell proliferation but induced a significant reduction of colonic apoptosis. The anti-apoptotic proteins Bcl-2 (B-cell lymphoma-2) and Bcl-xL (B-cell lymphoma extra large) were up-regulated in the mitochondria and down-regulated in the cytosol of the GADF mucosa, whereas the opposite was found for the pro-apoptotic protein Bax (Bcl-2-associated X protein), leading to an anti-apoptotic shift in the pattern of expression of the Bcl-2 family. Cytosolic cytochrome c and cleaved caspase-3 levels and caspase-3 activity were reduced by GADF. The modulation of the antioxidant enzyme system and the increase of the cytosolic GSH:glutathione disulfide (GSSG) ratio elicited by GADF helped to reduce oxidative damage. The cytosolic GSH:GSSG ratio was negatively related to apoptosis. These results indicate that GADF acts on the expression of the pro- and anti- apoptotic Bcl-2 proteins, attenuating the mitochondrial apoptotic pathway in the distal colonic mucosa. This effect appears to be associated with the antioxidant properties of GADF.
dc.description.departmentDepto. de Microbiología y Parasitología
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipSantander-Complutense University of Madrid
dc.description.sponsorshipSpanish Ministry of Science and Innovation, National R&D Programme
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/33601
dc.identifier.doi10.1017/S0007114512000517
dc.identifier.issn0007-1145
dc.identifier.officialurlhttp://dx.doi.org/10.1017/S0007114512000517
dc.identifier.relatedurlhttp://journals.cambridge.org/action/displayJournal?jid=BJN
dc.identifier.urihttps://hdl.handle.net/20.500.14352/44764
dc.journal.titleBritish journal of nutrition
dc.language.isoeng
dc.page.final16
dc.page.initial4
dc.publisherCambridge University Press
dc.relation.projectIDGR35/10-B-950574
dc.relation.projectIDAGL-2008-01633
dc.rights.accessRightsrestricted access
dc.subject.cdu579
dc.subject.keywordAntioxidant dietary fibre
dc.subject.keywordApoptosis
dc.subject.keywordRedox status
dc.subject.keywordColonic mucosa
dc.subject.ucmMicrobiología (Farmacia)
dc.subject.unesco3302.03 Microbiología Industrial
dc.titleGrape antioxidant dietary fibre prevents mitochondrial apoptotic pathways by enhancing Bcl-2 and Bcl-xL expression and minimising oxidative stress in rat distal colonic mucosa.
dc.typejournal article
dc.volume.number109
dspace.entity.typePublication
relation.isAuthorOfPublicationcd8bf5cb-359c-4c23-8574-f796f0061d6a
relation.isAuthorOfPublication82346295-96ca-4e6e-ad73-b70afa0b9f5b
relation.isAuthorOfPublication.latestForDiscovery82346295-96ca-4e6e-ad73-b70afa0b9f5b
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