Label-free quantitative 3D tomographic imaging for partially coherent light microscopy

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dc.contributor.authorSoto Rueda, Juan Manuel
dc.contributor.authorRodrigo Martín-Romo, José Augusto
dc.contributor.authorAlieva Krasheninnikova, Tatiana
dc.date.accessioned2023-06-17T22:12:23Z
dc.date.available2023-06-17T22:12:23Z
dc.date.issued2017-07-10
dc.description© 2017 Optical Society of America. Funding Ministerio de Economía y Competitividad (TEC2014-57394-P).
dc.description.abstractCrucial benefits provided by partially coherent light microscopy such as improved spatial resolution, optical sectioning and speckle-noise suppression are exploited here to achieve 3D quantitative imaging: reconstruction of the object refractive index (RI). We present a partially coherent optical diffraction tomography technique (PC-ODT) that can be easily implemented in commercially available bright-field microscopes. We show that the high numerical apertures of the objective and condenser lenses, together with optical refocusing, are main issues for achieving fast and successful 3D RI reconstruction of weak objects. In particular, the optical refocusing is performed by a high-speed focus tunable lens mounted in front of the digital camera enabling compatibility with commercial microscopes. The technique is experimentally demonstrated on different examples: diatom cells (biosilica shells), polystyrene micro-spheres and blood cells. The results confirm the straightforward 3D-RI reconstruction of the samples providing valuable quantitative information for their analysis. Thus, the PC-ODT can be considered as an efficient and affordable alternative to coherent ODT which requires specially designed holographic microscopes.
dc.description.departmentDepto. de Óptica
dc.description.facultyFac. de Ciencias Físicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/45310
dc.identifier.doi10.1364/OE.25.015699
dc.identifier.issn1094-4087
dc.identifier.officialurlhttp://dx.doi.org/10.1364/OE.25.015699
dc.identifier.relatedurlhttps://www.osapublishing.org
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18215
dc.issue.number14
dc.journal.titleOptics express
dc.language.isoeng
dc.page.final15712
dc.page.initial15699
dc.publisherOptical Soc Amer
dc.relation.projectIDTEC2014-57394-P
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu535
dc.subject.keywordOptical diffraction tomography
dc.subject.keywordPhase microscopy
dc.subject.keywordBlood-cells
dc.subject.keywordResolution
dc.subject.ucmÓptica (Física)
dc.subject.unesco2209.19 Óptica Física
dc.titleLabel-free quantitative 3D tomographic imaging for partially coherent light microscopy
dc.typejournal article
dc.volume.number25
dspace.entity.typePublication
relation.isAuthorOfPublication744b2ca4-d506-4370-b237-ee577fd591a0
relation.isAuthorOfPublicationf1512137-328a-4bb6-9714-45de778c1be4
relation.isAuthorOfPublication.latestForDiscovery744b2ca4-d506-4370-b237-ee577fd591a0
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