Magnetic Janus micromotors for fluorescence biosensing of tacrolimus in oral fluids

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dc.contributor.authorGlahn Martínez, Ana Bettina
dc.contributor.authorJurado-Sánchez, Beatriz
dc.contributor.authorBenito Peña, María Elena
dc.contributor.authorEscarpa, Alberto
dc.contributor.authorMoreno Bondi, María Cruz
dc.date.accessioned2024-01-16T11:22:20Z
dc.date.available2024-01-16T11:22:20Z
dc.date.issued2024
dc.description.abstractTacrolimus (FK506) is a macrolide lactone immunosuppressive drug that is commonly used in transplanted patients to avoid organ rejection. FK506 exhibits high inter- and intra-patient pharmacokinetic variability, making monitoring necessary for organ graft survival. This work describes the development of a novel bioassay for monitoring FK506. The bioassay is based on using polycaprolactone-based (PCL) magnetic Janus micromotors and a recombinant chimera receptor that incorporates the immunophilin tacrolimus binding protein 1A (FKBP1A) tagged with Emerald Green Fluorescent Protein (EmGFP). The approach relies on a fluorescence competitive bioassay between the drug and the micromotors decorated with a carboxylated FK506 toward the specific site of the fluorescent immunophilin. The proposed homogeneous assay could be performed in a single step without washing steps to separate the unbound receptor. The proposed approach fits the therapeutic requirements, showing a limit of detection of 0.8 ng/mL and a wide dynamic range of up to 90 ng/mL. Assay selectivity was evaluated by measuring the competitive inhibition curves with other immunosuppressive drugs usually co-administered with FK506. The magnetic propulsion mechanism allows for efficient operation in raw samples without damaging the biological binding receptor (FKBP1A-EmGFP). The enhanced target recognition and micromixing strategies hold considerable potential for FK506 monitoring in practical clinical use.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationGlahn-Martínez, Bettina, et al. «Magnetic Janus Micromotors for Fluorescence Biosensing of Tacrolimus in Oral Fluids». Biosensors and Bioelectronics, vol. 244, enero de 2024, p. 115796. https://doi.org/10.1016/j.bios.2023.115796.
dc.identifier.doi10.1016/j.bios.2023.115796
dc.identifier.issn0956-5663
dc.identifier.officialurlhttps://doi.org/10.1016/j.bios.2023.115796
dc.identifier.relatedurlhttps://www.sciencedirect.com/science/article/pii/S0956566323007388
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93325
dc.journal.titleBiosensors and Bioelectronics
dc.language.isoeng
dc.page.final8
dc.page.initial1
dc.publisherElsevier
dc.relation.projectIDRTI2018-096410-B-C21
dc.relation.projectIDPID2021-127457OBC21
dc.relation.projectIDPID2020-118154 GB-I00
dc.relation.projectIDTED2021132720B–I00
dc.relation.projectIDCM/JIN/ 2021-012 (B.J.S.)
dc.relation.projectIDTRANSNANOAVANSENS, S2018/NMT-4349 (A.E.)
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu543
dc.subject.keywordBioluminescent assay
dc.subject.keywordRecombinant protein
dc.subject.keywordTacrolimus
dc.subject.keywordTacrolimus binding protein 1A
dc.subject.keywordEmerald green fluorescent protein
dc.subject.keywordMicromotors
dc.subject.keywordJanus
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco23 Química
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco33 Ciencias Tecnológicas
dc.titleMagnetic Janus micromotors for fluorescence biosensing of tacrolimus in oral fluids
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number244
dspace.entity.typePublication
relation.isAuthorOfPublication1f584f9d-9c7c-4305-bfab-627d432f96fb
relation.isAuthorOfPublicationebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85
relation.isAuthorOfPublication8766057b-6628-4a02-a6db-20bddfaf3054
relation.isAuthorOfPublication.latestForDiscovery8766057b-6628-4a02-a6db-20bddfaf3054
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