Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation

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dc.contributor.authorIllescas Martínez, Beatriz María
dc.contributor.authorPérez Sánchez, Alfonso
dc.contributor.authorMallo, Araceli
dc.contributor.authorMartín Doménech, Ángel
dc.contributor.authorRodríguez Crespo, José Ignacio
dc.contributor.authorMartín León, Nazario
dc.date.accessioned2024-01-12T16:32:40Z
dc.date.available2024-01-12T16:32:40Z
dc.date.issued2020
dc.description.abstractNon-viral nucleic acid vectors able to display high transfection efficiencies with low toxicity and overcoming the multiple biological barriers are needed to further develop the clinical applications of gene therapy. The synthesis of hexakis-adducts of [60]fullerene endowed with 12, 24 and 36 positive ammonium groups and a tridecafullerene appended with 120 positive charges has been performed. The delivery of a plasmid containing the green fluorescent protein (EGFP) gene into HEK293 (Human Embryonic Kidney) cells resulting in effective gene expression has demonstrated the efficacy of these compounds to form polyplexes with DNA. Particularly, giant tridecafullerene macromolecules have shown higher efficiency in the complexation and transfection of DNA. Thus, they can be considered as promising non-viral vectors for transfection purposes.
dc.description.departmentDepto. de Química Orgánica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.statuspub
dc.identifier.citationIllescas, B. M.; Pérez-Sánchez, A.; Mallo, A.; Martín-Domenech, Á; Rodríguez-Crespo, I.; Martín, N. Multivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation. J. Mater. Chem. B 2020, 8, 4505-4515 DOI:10.1039/D0TB00113A.
dc.identifier.doi10.1039/d0tb00113a
dc.identifier.essn2050-7518
dc.identifier.issn2050-750X
dc.identifier.officialurlhttps://doi.org/10.1039/d0tb00113a
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92879
dc.issue.number20
dc.journal.titleJournal of Materials Chemistry B
dc.language.isoeng
dc.page.final4515
dc.page.initial4505
dc.publisherThe Royal Society of Chemistry
dc.rights.accessRightsmetadata only access
dc.subject.cdu547
dc.subject.ucmQuímica orgánica (Química)
dc.subject.unesco2306 Química Orgánica
dc.titleMultivalent cationic dendrofullerenes for gene transfer: synthesis and DNA complexation
dc.typejournal article
dc.volume.number8
dspace.entity.typePublication
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