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Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)

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dc.book.titleMicrobial steroids : methods and protocols
dc.contributor.authorGuevara, Govinda
dc.contributor.authorPerera González, Jesús
dc.contributor.authorNavarro Llorens, Juana María
dc.date.accessioned2023-06-18T00:22:16Z
dc.date.available2023-06-18T00:22:16Z
dc.date.issued2017
dc.description.abstractThin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/44684
dc.identifier.doi10.1007/978-1-4939-7183-1_24
dc.identifier.isbn978-1-4939-7182-4 (Print) 978-1-4939-7183-1 (Online)
dc.identifier.issn1064-3745, ESSN: 1940-6029
dc.identifier.officialurlhttps://link.springer.com/protocol/10.1007/978-1-4939-7183-1_24
dc.identifier.urihttps://hdl.handle.net/20.500.14352/19465
dc.issue.number1645
dc.journal.titleMethods in Molecular Biology
dc.language.isoeng
dc.page.final360
dc.page.initial347
dc.publication.placeNew York
dc.publisherSpringer
dc.relation.ispartofseriesMethods in molecular biology
dc.relation.projectIDPROBIODES (RTC- 2014-2249-1)
dc.rights.accessRightsrestricted access
dc.subject.cdu577.175.62
dc.subject.keywordResting cells
dc.subject.keywordDetection
dc.subject.keywordThin-layer chromatography
dc.subject.keywordTLC
dc.subject.keywordSteroids
dc.subject.keywordAD
dc.subject.keywordADD
dc.subject.keyword9OHAD
dc.subject.ucmBioquímica (Biología)
dc.subject.unesco2302 Bioquímica
dc.titleAnalysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)
dc.typebook part
dspace.entity.typePublication
relation.isAuthorOfPublicationd0b7aecd-194b-4ea4-b073-f5b1ec49a255
relation.isAuthorOfPublication.latestForDiscoveryd0b7aecd-194b-4ea4-b073-f5b1ec49a255

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