Two cholecystokinin receptor subtypes are identified in goldfish, being the CCKAR involved in the regulation of intestinal motility

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dc.contributor.authorTinoco Pérez, Ana Belén
dc.contributor.authorValenciano González, Ana Isabel
dc.contributor.authorGómez Boronat, Miguel
dc.contributor.authorBlanco, A.M.
dc.contributor.authorNisembaum, Laura Gabriela
dc.contributor.authorPedro Ormeño, Nuria de
dc.contributor.authorDelgado Saavedra, María Jesús
dc.date.accessioned2023-06-18T05:46:33Z
dc.date.available2023-06-18T05:46:33Z
dc.date.issued2015-09
dc.description.abstractCholecystokinin (CCK) plays a key role in the digestive physiology of vertebrates. However, very little is known about the role of CCK on intestinal functions in fish. The present study identifies two CCK receptor subtypes in a stomachless teleost, the goldfish (Carassius auratus), and investigates by using an in vitro system their involvement mediating the effects of the sulfated octapeptide of CCK (CCK-8S) on the motility of isolated proximal intestine. Partial-length mRNAs encoding two CCK receptor isoforms (CCKAR and CCKBR.I) were sequenced and the structural analysis showed that both receptors belong to the G-protein coupled receptor superfamily. Both gold- fish CCK receptor sequences were more closely related to zebrafish sequences, sharing the lowest similarities with cavefish and tilapia. The highest expression of goldfish CCKAR was observed along the whole intestine whereas the CCKBR gen was predominantly expressed in the hypothalamus, vagal lobe and posterior intestine. Application of CCK-8S to the organ bath evoked a concentration-dependent contractile response in intestine strips. The contractions were not blocked by either tetrodotoxin or atropine, suggesting that CCK-8S acts on the gut smooth muscle directly. Preincubations of intestine strips with devazepide and L365,260 (CCKAR and CCKBR receptor selective antagonists) showed that the CCK-8S-induced contraction could be partially mediated by the CCKAR receptor subtype, which is also the most abundant CCK receptor found in gastrointestinal tissues. In conclusion, two CCK receptors with a differential distribution pattern has been identified in goldfish, and the CCKAR subtype is mainly involved in the regulation of intestinal motility by the CCK-8S.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipMinisterio de Asuntos Exteriores y de Cooperación(España)-AECID
dc.description.sponsorshipMinisterio de Educación Cultura y Deporte (España)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/42501
dc.identifier.doi10.1016/j.cbpa.2015.05.027
dc.identifier.issn1095-6433
dc.identifier.officialurlhttp://www.sciencedirect.com/science/article/pii/S1095643315001622
dc.identifier.urihttps://hdl.handle.net/20.500.14352/23306
dc.journal.titleComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
dc.language.isoeng
dc.page.final201
dc.page.initial193
dc.publisherElsevier
dc.relation.projectID(AGL2013-46448-C3-2-R.)
dc.rights.accessRightsrestricted access
dc.subject.cdu597.5
dc.subject.cdu591.1
dc.subject.keywordCCK receptors
dc.subject.keywordCholecystokinin
dc.subject.keywordFish
dc.subject.keywordIntestinal motility
dc.subject.keywordOrgan bath
dc.subject.keywordPhylogeny
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmPeces
dc.subject.unesco2401.13 Fisiología Animal
dc.titleTwo cholecystokinin receptor subtypes are identified in goldfish, being the CCKAR involved in the regulation of intestinal motility
dc.typejournal article
dc.volume.number187
dspace.entity.typePublication
relation.isAuthorOfPublication58727d30-dd58-40f9-962a-9bc051bfc74c
relation.isAuthorOfPublication44ac4b2f-08dc-44a8-8d08-cea0a94715f7
relation.isAuthorOfPublication48071251-a236-4607-ab33-5332c1472628
relation.isAuthorOfPublication.latestForDiscovery58727d30-dd58-40f9-962a-9bc051bfc74c
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