The interplay of lung surfactant proteins and lipids assimilates the macrophage clearance of nanoparticles

Simple item page
dc.contributor.authorRuge, C.A.
dc.contributor.authorSchaefer, U.F.
dc.contributor.authorHerrmann, J.
dc.contributor.authorKirch, J.
dc.contributor.authorCañadas Benito, Olga
dc.contributor.authorEchaide Torreguitar, Mercedes
dc.contributor.authorPérez Gil, Jesús
dc.contributor.authorCasals Carro, María Jesús
dc.contributor.authorMüller, R.
dc.contributor.authorLehr, C.M.
dc.date.accessioned2024-01-30T15:11:48Z
dc.date.available2024-01-30T15:11:48Z
dc.date.issued2012
dc.description.abstractThe peripheral lungs are a potential entrance portal for nanoparticles into the human body due to their large surface area. The fact that nanoparticles can be deposited in the alveolar region of the lungs is of interest for pulmonary drug delivery strategies and is of equal importance for toxicological considerations. Therefore, a detailed understanding of nanoparticle interaction with the structures of this largest and most sensitive part of the lungs is important for both nanomedicine and nanotoxicology. Astonishingly, there is still little known about the bio-nano interactions that occur after nanoparticle deposition in the alveoli. In this study, we compared the effects of surfactant-associated protein A (SP-A) and D (SP-D) on the clearance of magnetite nanoparticles (mNP) with either more hydrophilic (starch) or hydrophobic (phosphatidylcholine) surface modification by an alveolar macrophage (AM) cell line (MH-S) using flow cytometry and confocal microscopy. Both proteins enhanced the AM uptake of mNP compared with pristine nanoparticles; for the hydrophilic ST-mNP, this effect was strongest with SP-D, whereas for the hydrophobic PL-mNP it was most pronounced with SP-A. Using gel electrophoretic and dynamic light scattering methods, we were able to demonstrate that the observed cellular effects were related to protein adsorption and to protein-mediated interference with the colloidal stability. Next, we investigated the influence of various surfactant lipids on nanoparticle uptake by AM because lipids are the major surfactant component. Synthetic surfactant lipid and isolated native surfactant preparations significantly modulated the effects exerted by SP-A and SP-D, respectively, resulting in comparable levels of macrophage interaction for both hydrophilic and hydrophobic nanoparticles. Our findings suggest that because of the interplay of both surfactant lipids and proteins, the AM clearance of nanoparticles is essentially the same, regardless of different intrinsic surface properties.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationRuge CA, Schaefer UF, Herrmann J, Kirch J, Cañadas O, Echaide M, Pérez-Gil J, Casals C, Müller R, Lehr CM. 2012. The interplay of lung surfactant proteins and lipids assimilates the macrophage clearance of nanoparticles. PLoS One. 2012; 7(7):e40775
dc.identifier.doi10.1371/journal.pone.0040775
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96634
dc.issue.number7
dc.journal.titlePLoS One
dc.language.isoeng
dc.page.initiale40775
dc.rights.accessRightsopen access
dc.subject.ucmCiencias
dc.subject.unesco23 Química
dc.subject.unesco24 Ciencias de la Vida
dc.titleThe interplay of lung surfactant proteins and lipids assimilates the macrophage clearance of nanoparticles
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number7
dspace.entity.typePublication
relation.isAuthorOfPublication25a89a2f-a381-4f15-9a6b-59430ee96a63
relation.isAuthorOfPublication9a800d46-4cd8-4a6d-97a1-4f2e9ce34f4e
relation.isAuthorOfPublicationbcddc7b1-6137-48ba-921d-4abd534dfd49
relation.isAuthorOfPublication85b288a3-1714-4210-becb-c6dc7e09f34a
relation.isAuthorOfPublication.latestForDiscovery25a89a2f-a381-4f15-9a6b-59430ee96a63
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PLoS One-2012.pdf
Size:
642.25 KB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos