The expression of GLP‐1 receptor mRNA and protein allows the effect of GLP‐1 on glucose metabolism in the human hypothalamus and brainstem

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dc.contributor.authorÁlvarez García, Elvira
dc.contributor.authorMartínez Ibáñez, María Dolores
dc.contributor.authorRoncero Rincón, Isabel
dc.contributor.authorChowen, Julie A.
dc.contributor.authorGarcía Cuartero, Beatriz
dc.contributor.authorGispert, Juan D.
dc.contributor.authorSanz Miguel, María Del Carmen
dc.contributor.authorVázquez Pérez, Patricia
dc.contributor.authorAntonio, Maldonado
dc.contributor.authorDe Cáceres, Javier
dc.contributor.authorDesco, Manuel
dc.contributor.authorPozo García, Miguel Ángel
dc.contributor.authorBlázquez Fernández, Enrique
dc.date.accessioned2024-01-29T15:33:08Z
dc.date.available2024-01-29T15:33:08Z
dc.date.issued2005
dc.description.abstractIn the present work, several experimental approaches were used to determine the presence of the glucagon-like peptide-1 receptor (GLP-1R) and the biological actions of its ligand in the human brain. In situ hybridization histochemistry revealed specific labelling for GLP-1 receptor mRNA in several brain areas. In addition, GLP-1R, glucose transporter isoform (GLUT-2) and glucokinase (GK) mRNAs were identified in the same cells, especially in areas of the hypothalamus involved in feeding behaviour. GLP-1R gene expression in the human brain gave rise to a protein of 56 kDa as determined by affinity cross-linking assays. Specific binding of 125I-GLP-1(7-36) amide to the GLP-1R was detected in several brain areas and was inhibited by unlabelled GLP-1(7-36) amide, exendin-4 and exendin (9-39). A further aim of this work was to evaluate cerebral-glucose metabolism in control subjects by positron emission tomography (PET), using 2-[F-18] deoxy-D-glucose (FDG). Statistical analysis of the PET studies revealed that the administration of GLP-1(7-36) amide significantly reduced (p < 0.001) cerebral glucose metabolism in hypothalamus and brainstem. Because FDG-6-phosphate is not a substrate for subsequent metabolic reactions, the lower activity observed in these areas after peptide administration may be due to reduction of the glucose transport and/or glucose phosphorylation, which should modulate the glucose sensing process in the GLUT-2- and GK-containing cells.en
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationAlvarez E, Martínez MD, Roncero I, et al. The expression of GLP-1 receptor mRNA and protein allows the effect of GLP-1 on glucose metabolism in the human hypothalamus and brainstem. J Neurochem. 2005;92(4):798-806. doi:10.1111/j.1471-4159.2004.02914.x
dc.identifier.doi10.1111/j.1471-4159.2004.02914.x
dc.identifier.issn0022-3042
dc.identifier.issn1471-4159
dc.identifier.officialurlhttps://doi.org/10.1111/j.1471-4159.2004.02914.x
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96176
dc.issue.number4
dc.journal.titleJournal of Neurochemistry
dc.language.isoeng
dc.page.final806
dc.page.initial798
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu612.017
dc.subject.keywordBiological effects
dc.subject.keywordGene expression
dc.subject.keywordGlucagon-likepeptide-1 receptor
dc.subject.keywordGlucose sensing
dc.subject.keywordHuman brain
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleThe expression of GLP‐1 receptor mRNA and protein allows the effect of GLP‐1 on glucose metabolism in the human hypothalamus and brainstemen
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number92
dspace.entity.typePublication
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