Identification of Major Allergens in Watermelon

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dc.contributor.authorPastor Vargas, Carlos
dc.contributor.authorCuesta-Herranz, Javier
dc.contributor.authorCases, Barbara
dc.contributor.authorPérez-Gordo, Marina
dc.contributor.authorFigueredo, Elena
dc.contributor.authorHeras González, Manuel De Las
dc.contributor.authorVivanco Martínez, Fernando
dc.date.accessioned2024-01-15T12:55:59Z
dc.date.available2024-01-15T12:55:59Z
dc.date.issued2009
dc.description.abstractBackground: Watermelon is a worldwide consumed Cucurbitaceae fruit that can elicit allergic reactions. However, the major allergens of watermelon are not known. The aim of this study is to identify and characterize major allergens in watermelon. Methods: Twenty-three patients allergic to watermelon took part in the study. The diagnosis was based on a history of symptoms and positive skin prick-prick tests to watermelon, confirmed by positive open oral challenge testing to watermelon pulp. Allergenic components were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by N-terminal amino acid sequencing and mass spectrometry. Allergens were purified combining several chromatographic steps. Results: Several IgE binding bands (8-120 kDa) were detected in watermelon extract. Three major allergens were identified as malate dehydrogenase (36 kDa), triose phosphate isomerase (28 kDa) and profilin (13 kDa). Purified allergens individually inhibited IgE binding to the whole watermelon extract. Conclusions: All in all these results indicate that malate dehydrogenase, triose phosphate isomerase and profilin are major allergens involved in watermelon allergy.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Educación y Ciencia (España)
dc.description.sponsorshipVegetalia Network
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statuspub
dc.identifier.citationPastor C, Cuesta-Herranz J, Cases B, Pérez-Gordo M, Figueredo E, de las Heras M, Vivanco F. Identification of major allergens in watermelon. Int Arch Allergy Immunol. 2009;149(4):291-8. doi: 10.1159/000205574. Epub 2009 Mar 17. PMID: 19295232.
dc.identifier.doi10.1159/000205574
dc.identifier.essn1423-0097
dc.identifier.issn1018-2438
dc.identifier.officialurlhttps://doi.org/10.1159/000205574
dc.identifier.pmid19295232
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93097
dc.issue.number4
dc.journal.titleInternational Archives on Allergy & Immunology
dc.language.isoeng
dc.page.final298
dc.page.initial291
dc.publisherKarger
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu577.1
dc.subject.keywordMalate dehydrogenase
dc.subject.keywordTriose phosphate isomerase
dc.subject.keywordProfilin
dc.subject.keywordWatermelon allergy
dc.subject.keywordFood allergy
dc.subject.ucmAlergología
dc.subject.unesco2412.05 Hipersensibilidad
dc.titleIdentification of Major Allergens in Watermelon
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number149
dspace.entity.typePublication
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relation.isAuthorOfPublication91270072-7876-41d0-836e-3bd33f7b7f96
relation.isAuthorOfPublication6f3e7679-cbc7-4f23-8355-2de0876d46ad
relation.isAuthorOfPublication.latestForDiscovery25af78c7-0077-4891-a14e-bcd8e51fe408
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