Hirsutellin A: A Paradigmatic Example of the Insecticidal Function of Fungal Ribotoxins

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dc.contributor.authorHerrero Galán, Elías
dc.contributor.authorGarcía Ortega, Lucía
dc.contributor.authorLacadena García-Gallo, Francisco Javier
dc.contributor.authorOlombrada Sacristán, Miriam
dc.contributor.authorMartínez Del Pozo, Álvaro
dc.contributor.authorGavilanes Franco, José Gregorio
dc.contributor.authorOñaderra Sánchez, Mercedes
dc.date.accessioned2023-06-19T13:44:48Z
dc.date.available2023-06-19T13:44:48Z
dc.date.issued2013-07-09
dc.description.abstractThe fungal pathogen Hirsutella thompsonii produces an insecticidal protein named hirsutellin A (HtA), which has been described to be toxic to several species of mites, insect larvae, and cells. On the other hand, on the basis of an extensive biochemical and structural characterization, HtA has been considered to be a member of the ribotoxins family. Ribotoxins are fungal extracellular ribonucleases, which inactivate ribosomes by specifically cleaving a single phosphodiester bond located at the large rRNA. Although ribotoxins were brought to light in the 1960s as antitumor agents, their biological function has remained elusive. Thus, the consideration of hirsutellin A, an insecticidal protein, as a singular ribotoxin recalled the idea of the biological activity of these toxins as insecticidal agents. Further studies have demonstrated that the most representative member of the ribotoxin family, α-sarcin, also shows strong toxic action against insect cells. The determination of high resolution structures, the characterization of a large number of mutants, and the toxicity assays against different cell lines have been the tools used for the study of the mechanism of action of ribotoxins at the molecular level. The aim of this review is to serve as a compilation of the facts that allow identification of HtA as a paradigmatic example of the insecticidal function of fungal ribotoxins.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICIIN)
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/66291
dc.identifier.doi10.3390/insects4030339
dc.identifier.issn2075-4450
dc.identifier.officialurlhttps://doi.org/10.3390/insects4030339
dc.identifier.relatedurlhttps://www.mdpi.com/2075-4450/4/3/339
dc.identifier.urihttps://hdl.handle.net/20.500.14352/34362
dc.issue.number3
dc.journal.titleInsects
dc.language.isoeng
dc.page.final356
dc.page.initial339
dc.publisherMDPI
dc.relation.projectIDBFU2009-10185 y BFQ 2012-32404
dc.relation.projectIDESFUNPROT-UCM
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordinsecticidal proteins
dc.subject.keywordribotoxins
dc.subject.keyworda-sarcin
dc.subject.keywordhirsutellin
dc.subject.keywordribonucleases
dc.subject.keywordcytotoxic proteins
dc.subject.ucmBioquímica (Química)
dc.titleHirsutellin A: A Paradigmatic Example of the Insecticidal Function of Fungal Ribotoxins
dc.typejournal article
dc.volume.number4
dspace.entity.typePublication
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