Signalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress.

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dc.contributor.authorGarcía, Raúl
dc.contributor.authorPulido, Verónica
dc.contributor.authorOrellana Muñoz, Sara
dc.contributor.authorNombela, César
dc.contributor.authorVázquez de Aldana, Carlos R.
dc.contributor.authorRodríguez Peña, José Manuel
dc.contributor.authorArroyo, Javier
dc.date.accessioned2023-06-17T13:24:03Z
dc.date.available2023-06-17T13:24:03Z
dc.date.issued2019-02-28
dc.description.abstractPost-transcriptional control of mRNA is a key event in the regulation of gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role in this process, particularly under stress conditions. In this work, we show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures. This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. Remarkably, P-body assembly requires the catalytic activity of the MAPK of the pathway, Slt2/Mpk1. In accordance with the control exerted by this signalling pathway, the timing of P-body formation is similar to that of the activation of the CWI pathway. Noticeably, mRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation. Moreover, when these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that they play a role in the mRNA lifecycle under stress conditions.
dc.description.departmentDepto. de Microbiología y Parasitología
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/55659
dc.identifier.doi10.1038/s41598-019-40112-9
dc.identifier.issn2045-2322
dc.identifier.officialurlhttps://www.nature.com/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/13371
dc.issue.number1
dc.journal.titleScientific reports
dc.language.isoeng
dc.page.initial3186
dc.publisherNature Research
dc.relation.projectID(BIO2013-48136-P; BIO2016-79289-P; BIO2015-70195-C2-1-R)
dc.relation.projectIDInGEMICS-CM (B2017/BMD3691)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu579
dc.subject.cdu576
dc.subject.ucmBiología celular (Farmacia)
dc.subject.ucmMicrobiología (Farmacia)
dc.subject.unesco3302.03 Microbiología Industrial
dc.titleSignalling through the yeast MAPK Cell Wall Integrity pathway controls P-body assembly upon cell wall stress.
dc.typejournal article
dc.volume.number9
dspace.entity.typePublication
relation.isAuthorOfPublication85b0cb43-60bf-4823-ae50-8bd0ca14731a
relation.isAuthorOfPublication.latestForDiscovery85b0cb43-60bf-4823-ae50-8bd0ca14731a
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