Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation

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dc.contributor.authorGarcía-Sánchez, Marta
dc.contributor.authorJiménez-Pelayo, Laura
dc.contributor.authorRegidor-Cerrillo, Javier
dc.contributor.authorOsoro, Koldo
dc.contributor.authorVázquez Arbáizar, María Patricia
dc.contributor.authorHorcajo Iglesias, María Del Pilar
dc.contributor.authorJiménez Meléndez, Alejandro
dc.contributor.authorOrtega Mora, Luis Miguel
dc.contributor.authorCollantes Fernández, Esther
dc.date.accessioned2024-02-02T16:51:07Z
dc.date.available2024-02-02T16:51:07Z
dc.date.issued2021-06-22
dc.description.abstractBovine neosporosis is currently considered one of the main causes of abortion in cattle worldwide and the outcome of the infection is, in part, determined by Neospora caninum isolate virulence. However, the dam and foetal immune responses associated with this factor are largely unknown. We used a model of bovine infection at day 110 of gestation to study the early infection dynamics (10- and 20-days post-infection, dpi) after experimental challenge with high- and low-virulence isolates of N. caninum (Nc-Spain7 and Nc-Spain1H, respectively). In the present work, dam peripheral cellular immune responses were monitored twice a week from -1 to 20 dpi. At different time points, IFN-γ and IL-4 production was investigated in stimulated dam blood and the percentage of monocytes, NK cells, B cells and T cells (CD4+, CD8+ and γδ) in peripheral blood mononuclear cells (PBMC) were determined by flow cytometry. In addition, maternal iliofemoral lymph nodes and foetal spleen and thymus were collected at 10 and 20 dpi for the study of the same cell subpopulations. Peripheral immune response dynamics were similar after the infection with both isolates, with a significant increase in the percentage of CD4+ T cells at 6 and 9 dpi in PBMC, coincident with the higher levels of IFN-γ and IL-4 release. However, the levels of IFN-γ were significantly higher and an increase in CD8+ T cells at 9, 13 and 20 dpi was observed in the dams infected with Nc-Spain7. Nc-Spain1H infection induced higher IL4 levels in stimulated blood and a higher CD4+/CD8+ ratio in PBMC. The analysis of the maternal iliofemoral lymph node showed a significant enhancement in the percentage of NK, CD4+ and CD8+ T cells for the animals infected with the highly virulent isolate and euthanized at 20 dpi. Regarding the foetal responses, the most remarkable result was an increase in the percentage of monocytes at 20 dpi in the spleen of foetuses from both infected groups, which suggests that foetuses were able to respond to N. caninum infection at mid gestation. This work provides insights into how isolate virulence affects the maternal and foetal immune responses generated against N. caninum, which may influence the course of infection.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMINECO
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.doi10.3389/fcimb.2021.684670
dc.identifier.issn2235-2988
dc.identifier.officialurlhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.684670/full
dc.identifier.pmid34239816
dc.identifier.relatedurlhttps://pubmed.ncbi.nlm.nih.gov/34239816/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/98444
dc.issue.number684670
dc.journal.titleFrontiers in Cellular and Infection Microbiology
dc.language.isoeng
dc.publisherFrontiers Media S.A.
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//BES-2014-070723/ES/BES-2014-070723/
dc.relation.projectIDPLATESA2-CM P2018/BAA-4370
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2013-44694-R/ES/VIRULENCIA EN LA NEOSPOROSIS BOVINA: ESTUDIOS IN VIVO E IN VITRO DE LA INTERACCION PARASITO-HOSPEDADOR CON AISLADOS DE BAJA%2FALTA VIRULENCIA/
dc.relation.projectIDAGL2016-75935-C2-1-R
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu61
dc.subject.keywordcellular immune responses
dc.subject.keywordNeospora caninum
dc.subject.keywordpathogenesis
dc.subject.keywordpregnant cattle
dc.subject.keywordvirulence
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleMaternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number11
dspace.entity.typePublication
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relation.isAuthorOfPublication29b2edc5-34bb-428d-90c9-ec9664ba0bbe
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Maternal and Foetal Cellular Immune Responses in Dams Infected With High- and Low- Virulence Isolates of Neospora caninum at Mid-Gestation
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