RYS1, a foldback transposon, is activated by tissue culture and shows preferential insertion points into the rye genome

Alves E.; Linacero De La Fuente, M. Rosario; Ballesteros Redondo, María Isabel; Vázquez A.M.

RYS1, a foldback transposon, is activated by tissue culture and shows preferential insertion points into the rye genome

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Official URL

https://link.springer.com/article/10.1007/s00122-005-2013-9

Publication date

2005

Authors

Alves E.

Linacero De La Fuente, M. Rosario

Ballesteros Redondo, María Isabel

Vázquez A.M.

Publisher

Springer

Citations

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URI

https://hdl.handle.net/20.500.14352/91854

Abstract

The study of two variable amplicons of rye indicates that RYS1, a mobile element, is activated during tissue culture. We propose that RYS1 could be a foldback (FB) transposon. The FB transposons have been rarely reported in plants; RYS1 is the first described in rye and also the first active plant FB transposon reported. Preferential integration points in the rye genome exist, because the new insertions seem to be located, in all studied cases, in the same genome positions. We assume that RYS1 became active in rye very recently, as different plants from in vivo-growing cultivars showed that these elements were present or absent in the same genomic position in which the in vitro-activated element was found. This high rate of modification in these particular loci, both in the in vivo and in vitro populations, could indicate that probably the mechanisms promoting genetic variability in nature are the same that induce variation in vitro, and the modifications induced by somaclonal variation could be already present in vivo populations.