Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen

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dc.contributor.authorZabala Argüelles, Sonsoles Mercedes
dc.contributor.authorSerres Dalmau, María Consolacion
dc.contributor.authorMontero Serra, Natalia
dc.contributor.authorCrespo Castejón, Francisco
dc.contributor.authorLorenzo González, Pedro Luis
dc.contributor.authorPérez-Aguilera, Verónica
dc.contributor.authorGalán López, Carmen
dc.contributor.authorDomínguez Gimbernat, Mónica
dc.contributor.authorOliet Palá, Agustín
dc.contributor.authorMoreno, Santiago
dc.contributor.authorGonzález Zorn, Bruno
dc.contributor.authorGutiérrez Cepeda, Luna
dc.date.accessioned2024-04-02T10:58:45Z
dc.date.available2024-04-02T10:58:45Z
dc.date.issued2024-01-05
dc.descriptionAuthor Contributions: The study was conceived by L.G.-C. and C.S. who, together with S.M.Z., B.G.-Z., P.L.L., S.M. and F.C., participated in its design and coordination. S.M.Z., L.G.-C., V.P.-A., M.D.-G., C.G. and F.C. performed the semen collection, and processed the samples for refrigeration. Semen evaluation was executed by S.M.Z., V.P.-A., A.O., C.G., M.D.-G., F.C. and L.G.-C. Microbial load assessment was conducted by B.G.-Z. and N.M. with the assistance of S.M.Z. and V.P.-A. for sample preparation. L.G.-C. and C.S. performed the statistical analysis, with the support of Pedro Cuesta. C.S., L.G.-C., S.M.Z., B.G.-Z., P.L.L., F.C. and S.M. interpreted the data. The manuscript was written by L.G.-C., S.M.Z., C.S. and F.C., helped by the rest of authors, who revised it critically. All authors have read and approved the final version of the manuscript and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work have been appropriately investigated and resolved. All authors have read and agreed to the published version of the manuscript.
dc.description.abstractThe study assessed the impact of four equine semen processing techniques on sperm quality and microbial load immediately post-processing and after 48 h of refrigeration. The aim was to explore the potential reduction of prophylactic antibiotic usage in semen extenders. Semen from ten adult stallions was collected and processed under a strict hygiene protocol and divided into four aliquots: Simple Centrifugation with antibiotics (SC+), Simple Centrifugation (SC−), Single-Layer Colloidal Centrifugation (CC−), and Filtration (with SpermFilter®) (F−), all in extenders without antibiotics. Sperm motility, viability, and microbial load on three culture media were assessed. No significant differences were observed in the main in the sperm quality parameters among the four protocols post-processing and at 48 h (p < 0.05 or p < 0.1). Microbial loads in Columbia 5% Sheep Blood Agar and Schaedler vitamin K1 5% Sheep Blood Agar mediums were significantly higher (p < 0.10) for raw semen than for CS+, CC−, and F− post-processing. For Sabouraud Dextrose Agar medium, the microbial load was significantly higher (p < 0.10) in raw semen compared to CS+ and F−. No significant differences (p < 0.10) were found in 48 h chilled samples. Regardless of antibiotic presence, the evaluated processing methods, when combined with rigorous hygiene measures, maintained semen quality and reduced microbial load to the same extent as a traditional protocol using antibiotics.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipInstituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario
dc.description.statuspub
dc.identifier.citationZabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Perez-Aguilera V, Galan C, Dominguez-Gimbernat M, Oliet A, Moreno S, Gonzalez-Zorn B and Gutierrez-Cepeda L*. Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen. Animals, 14(2):179. 2024. (A). ISSN: 2076-2615. Impact factor 2022: 3.000. Category: Agriculture, dairy & animal science, Quartile: 1, Position: 12 of 62. DOI: 10.3390/ani14020179
dc.identifier.doi10.3390/ani14020179
dc.identifier.issn2076-2615
dc.identifier.officialurl10.3390/ani14020179
dc.identifier.urihttps://hdl.handle.net/20.500.14352/102498
dc.issue.number179
dc.journal.titleAnimals
dc.language.isoeng
dc.publisherMDPI
dc.relation.projectID5
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordchilled equine semen
dc.subject.keywordsemen microbial load
dc.subject.keywordsimple centrifugation
dc.subject.keywordsingle-layer colloidal centrifugation
dc.subject.keywordfiltration
dc.subject.keywordabsence of antibiotics
dc.subject.keywordantibiotics-free extender
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleStrategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number14
dspace.entity.typePublication
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