Characterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin

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dc.contributor.authorOlombrada, Miriam
dc.contributor.authorMedina, Pilar
dc.contributor.authorBudia, Flor
dc.contributor.authorGavilanes, José G.
dc.contributor.authorMartínez Del Pozo, Álvaro
dc.contributor.authorGarcía Ortega, Lucía
dc.date.accessioned2023-06-17T23:53:16Z
dc.date.available2023-06-17T23:53:16Z
dc.date.issued2016-09
dc.description.abstractMetarhizium anisopliae is an entomopathogenic fungus relevant in biotechnology with applications like malaria vector control. Studies of its virulence factors are therefore of great interest. Fungal ribotoxins are toxic ribonucleases with extraordinary efficiency against target ribosomes and suggested as potential insecticides. Here, we describe this ribotoxin characteristic activity in M. anisopliae cultures. Anisoplin has been obtained as a recombinant protein and further characterized. It is structurally similar to hirsutellin A, the ribotoxin from the entomopathogen Hirsutella thompsonii. Moreover, anisoplin shows the ribonucleolytic activity typical of ribotoxins and cytotoxicity against insect cells. How Metarhizium uses this toxin and possible applications are on perspective.
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Biológicas
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedFALSE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statussubmitted
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39181
dc.identifier.doi10.1515/hsz-2016-0119
dc.identifier.issn(Online) 1437-4315, ISSN (Print) 1431-6730
dc.identifier.officialurlhttp://www.degruyter.com/view/j/bchm.ahead-of-print/hsz-2016-0119/hsz-2016-0119.xml
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18970
dc.journal.titleBiological Chemistry
dc.language.isoeng
dc.publisherDe Gruyter
dc.relation.projectIDBFU2012-32404 and AGL2010-22196-C02-02
dc.relation.projectIDESFUNPROT-UCM
dc.rights.accessRightsrestricted access
dc.subject.cdu577.1
dc.subject.keywordhirsutellin
dc.subject.keywordinsecticide
dc.subject.keywordribonuclease
dc.subject.keywordribotoxin
dc.subject.keywordsarcin.
dc.subject.ucmBioquímica (Química)
dc.titleCharacterization of a new toxin from the entomopathogenic fungus Metarhizium anisopliae: the ribotoxin anisoplin
dc.typejournal article
dspace.entity.typePublication
relation.isAuthorOfPublication4d35a8a6-8bd3-4ff4-b179-57581d8d36d8
relation.isAuthorOfPublicationb8f84062-84af-45de-876d-9ee1b31aa47a
relation.isAuthorOfPublication.latestForDiscoveryb8f84062-84af-45de-876d-9ee1b31aa47a
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