A vaccine formulation combining rhoptry proteins NcROP40 and NcROP2 improves pup survival in a pregnant mouse model of neosporosis

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dc.contributor.authorPastor Fernández, Iván
dc.contributor.authorArranz-Solís, David
dc.contributor.authorRegidor-Cerrillo, Javier
dc.contributor.authorÁlvarez García, Gema
dc.contributor.authorHemphill, Andrew
dc.contributor.authorGarcía Culebras, Alicia
dc.contributor.authorCuevas-Martín, Carmen
dc.contributor.authorOrtega Mora, Luis Miguel
dc.date.accessioned2023-12-15T18:46:12Z
dc.date.available2023-12-15T18:46:12Z
dc.date.issued2015-01-30
dc.description.abstractCurrently there are no effective vaccines for the control of bovine neosporosis. During the last years several subunit vaccines based on immunodominant antigens and other proteins involved in adhesion, invasion and intracellular proliferation of Neospora caninum have been evaluated as targets for vaccine development in experimental mouse infection models. Among them, the rhoptry antigen NcROP2 and the immunodominant NcGRA7 protein have been assessed with varying results. Recent studies have shown that another rhoptry component, NcROP40, and NcNTPase, a putative dense granule antigen, exhibit higher expression levels in tachyzoites of virulent N. caninum isolates, suggesting that these could be potential vaccine candidates to limit the effects of infection. In the present work, the safety and efficacy of these recombinant antigens formulated in Quil-A adjuvant as monovalent vaccines or pair-wise combinations (rNcROP40 + rNcROP2 and rNcGRA7 + rNcNTPase) were evaluated in a pregnant mouse model of neosporosis. All the vaccine formulations elicited a specific immune response against their respective native proteins after immunization. Mice vaccinated with rNcROP40 and rNcROP2 alone or in combination produced the highest levels of IFN-γ and exhibited low parasite burdens and low IgG antibody levels after the challenge. In addition, most of the vaccine formulations were able to increase the median survival time in the offspring. However, pup survival only ensued in the groups vaccinated with rNcROP40 + rNcROP2 (16.2%) and rNcROP2 (6.3%). Interestingly, vertical transmission was not observed in those survivor pups immunized with rNcROP40 + rNcROP2, as shown by PCR analyses. These results show a partial protection against N. caninum infection after vaccination with rNcROP40 + rNcROP2, suggesting a synergistic effect of the two recombinant rhoptry antigens.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCAI Ciencias de la Tierra y Arqueometría
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.doi10.1016/j.vetpar.2014.12.009
dc.identifier.essn1873-2550
dc.identifier.issn0304-4017
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S030440171400630X?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/91382
dc.issue.number3-4
dc.journal.titleVeterinary Parasitology
dc.language.isoeng
dc.page.final215
dc.page.initial203
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordNeospora caninum-vaccine
dc.subject.keywordNcROP40
dc.subject.keywordNcROP2
dc.subject.keywordNcGRA7
dc.subject.keywordNcNTPase
dc.subject.keywordPregnant mouse model
dc.subject.ucmParasitología (Veterinaria)
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleA vaccine formulation combining rhoptry proteins NcROP40 and NcROP2 improves pup survival in a pregnant mouse model of neosporosis
dc.typejournal article
dc.volume.number207
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery61620b46-f8b9-43cb-bce0-ca70479895da
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