Bioactive polyphenols from Ranunculus macrophyllus Desf. Roots: quantification, identification and antioxidant activity

Simple item page
dc.contributor.authorDeghima, Amirouche
dc.contributor.authorRighi, Nadjat
dc.contributor.authorRosales Conrado, Noelia
dc.contributor.authorLeón González, María Eugenia De
dc.contributor.authorGómez Mejía, Esther
dc.contributor.authorMadrid Albarrán, María Yolanda
dc.contributor.authorBaali, Faiza
dc.contributor.authorBedjou, Fatiha
dc.date.accessioned2024-01-16T12:25:26Z
dc.date.available2024-01-16T12:25:26Z
dc.date.issued2020-08-01
dc.description.abstractRanunculus macrophyllus Desf. is an Algerian medicinal plant whose roots are used in traditional medicine to cure feminine infertility and other diseases, however there are no studies regarding its phytochemistry and biological activities. The aim of this work is to study the phytochemical composition and antioxidant activity of different solvents fractions from the roots of Ranunculus macrophyllus Desf. Spectrophotometric and chromatographic methods were used to study the phytochemical composition; while antiradical, iron chelating ability, reducing power and lipid peroxidation were studied in-vitro. The ethyl acetate fraction showed the highest values of total phenolic compounds (271.0 ± 0.2 µg GAE/mg dry extract (d.e), flavonols (24 ± 5 µg RE/ mg d.e) and condensed tannins (129 ± 10 µg CE/mg d.e), while the hexane fraction contained the highest amount of triterpenoids (91 ± 7 µg UAE/mg d.e). The highest radical scavenging ability was recorded for the ethyl acetate fraction against DPPH (IC50 = 3.7 ± 0.1 µg/mL) and ABTS (IC50 = 81 ± 3 µg/mL) whereas the hexane fraction had the best hydrogen peroxide radical scavenging (IC50 = 380 ± 4 µg/mL). The ethyl acetate fraction had the best total antioxidant capacity (TAC = 361 ± 1 µAAE/ mg extract) and reducing power (310 ± 2 µAAE/mg extract). The β-carotene bleaching was inhibited at high rate even after 24 h by the ethyl acetate fraction (81.0 ± 0.5 %). All activities were correlated with the polyphenolic content of the fractions. Capillary LC-DAD and LC-MS/MS analysis of ethyl acetate fraction revealed high amounts of gallic acid (9.3 ± 0.6 mg/g d.e), dihydroxybenzoic acid (8.1 ± 0.2 mg/g d.e) and hesperidin (5.9 ± 0.6 mg/g d.e). With such high amounts of polyphenols and strong antioxidant activity Ranunculus macrophyllus Desf. roots could have a potential use in pharmaceutical and nutraceutical industries.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipCommunity of Madrid/FEDER program
dc.description.sponsorshipMinistry of Economy and Competitiveness
dc.description.sponsorshipComplutense University
dc.description.statuspub
dc.identifier.citationDeghima, A; Bedjou, F; Righi, N; Baali, F; Rosales-Conrado, N; Leon-Gonzalez, ME; Gomez-Mejia, E; Madrid, Y. Bioactive polyphenols from Ranunculus macrophyllus Desf. Roots: Quantification, identification and antioxidant activity. 2020, 132: 204-214
dc.identifier.doi10.1016/j.sajb.2020.03.036
dc.identifier.issn0254-6299
dc.identifier.officialurlhttps://www.journals.elsevier.com/south-african-journal-of-botany
dc.identifier.relatedurlhttp://dx.doi.org/10.1016/j.sajb.2020.03.036
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93361
dc.journal.titleSouth African Journal of Botany
dc.language.isoeng
dc.page.final214
dc.page.initial204
dc.publisherElsevier
dc.relation.projectIDS2018/BAA-4393, AVANSECAL-II-CM
dc.relation.projectIDCTQ 2017-83569-C2-1-R]
dc.relation.projectIDCT17/ 17-CT18/17]
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordRanunculus macrophyllus Desf
dc.subject.keywordRoots
dc.subject.keywordPolyphenols
dc.subject.keywordAntioxidant
dc.subject.keywordAntiradical
dc.subject.keywordTriterpenoids
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleBioactive polyphenols from Ranunculus macrophyllus Desf. Roots: quantification, identification and antioxidant activity
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number132
dspace.entity.typePublication
relation.isAuthorOfPublicatione5625011-ba32-4a14-9ba0-c9305cb5d68e
relation.isAuthorOfPublication90a102b1-a583-41ce-a218-5ece43e45469
relation.isAuthorOfPublication719857fa-0325-4a81-b30a-199fd79c68c0
relation.isAuthorOfPublication190287de-ed47-4954-86ff-d4f1eddff035
relation.isAuthorOfPublication.latestForDiscoverye5625011-ba32-4a14-9ba0-c9305cb5d68e
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
South African Journal of Botany.pdf
Size:
969.43 KB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos