Antimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices

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dc.contributor.authorGalán-Relaño, Ángela
dc.contributor.authorGómez-Gascón, Lidia
dc.contributor.authorLuque, Inmaculada
dc.contributor.authorBarrero-Dominguez, Belén
dc.contributor.authorCardoso-Toset, Fernando
dc.contributor.authorTarradas, Carmen
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.contributor.authorVela Alonso, Ana Isabel
dc.contributor.authorCasamayor Sanz, Almudena
dc.date.accessioned2024-01-19T10:55:53Z
dc.date.available2024-01-19T10:55:53Z
dc.date.issued2019
dc.description.abstractTrueperella pyogenes is an opportunistic pathogen associated with a variety of diseases and responsible for important economic losses for pig production. Minimal Inhibitory Concentration (MIC) and Pulsed Field Gel Electrophoresis (PFGE) typing analysis were used to determine the MIC distribution and to genetically characterize a total of 180 T. pyogenes isolates obtained from slaughtered pigs reared under intensive (TpIN, n = 89) and extensive (TpEX, n = 91) farming practices. Low MIC90 values for penicillin and amoxicillin (0.008 and 0.06 μg/ml, respectively), ceftiofur, gentamicin and enrofloxacin (1 μg/ml, respectively) were obtained, so they could be of choice for the empiric treatment of T. pyogenes infections. Except for the penicillin, amoxicillin and ceftiofur, a statistically significant difference was observed in the MIC distribution of all antimicrobials analysed between TpIN and TpEX isolates. Also, MIC90 values were higher in TpIN than in TpEX isolates for neomycin and streptomycin (32 μg/ml vs 8 μg/ml), sulfamethoxazole/trimethoprim (30.4/1.6 μg/ml vs 1.90/0.10 μg/ml) and tylosin (≥1024 μg/ml vs 1 μg/ml). A relatively lower genetic diversity was detected in TpIN in comparison with TpEX isolates (GD 0.42 and GD 0.47, respectively). All isolates were distributed in three clusters (A, B, C). TpIN isolates were statistically associated with cluster A (P = 0.0002; OR 3.21; CI95 1.74–5.93), whereas the TpEX were distributed throughout the dendrogram, showing more genetic diversity. These data suggest that the antimicrobial susceptibility and genetic variability of the T. pyogenes isolates could be influenced by the management systems.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Agricultura, Pesca y Alimentación (20140020001824)
dc.description.statuspub
dc.identifier.doiDOI: 10.1016/j.vetmic.2019.04.011
dc.identifier.essn1873-2542
dc.identifier.issn1873-2542
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0378113518308563?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94011
dc.journal.titleVeterinary Microbiology
dc.language.isoeng
dc.page.final95
dc.page.initial89
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordPigs
dc.subject.keywordManagement sytems
dc.subject.keywordTrueperella pyogenes
dc.subject.keywordMIC
dc.subject.keywordPFGE
dc.subject.ucmSanidad animal
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleAntimicrobial susceptibility and genetic characterization of Trueperella pyogenes isolates from pigs reared under intensive and extensive farming practices
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number232
dspace.entity.typePublication
relation.isAuthorOfPublicationbaae9555-7eff-4de2-88b8-ad1c8bf8dd98
relation.isAuthorOfPublication83481d48-83ea-472f-8b74-067bc00debee
relation.isAuthorOfPublication1662e54a-b782-434d-8eba-4d902d6b51d9
relation.isAuthorOfPublication.latestForDiscoverybaae9555-7eff-4de2-88b8-ad1c8bf8dd98
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