Base de datos Cytokine analysis to evaluate severity in primary open-angle glaucoma and exfoliation glaucoma

No Thumbnail Available
Official URL
Full text at PDC
Publication Date
Advisors (or tutors)
Journal Title
Journal ISSN
Volume Title
Google Scholar
Research Projects
Organizational Units
Journal Issue
Purpose: To analyze cytokines in tear and aqueous humour as biomarkers for evaluation of severity of glaucoma. Design: Cross-sectional study Subjects: Tear and aqueous humour samples were collected from 15 exfoliative glaucoma (XFG) patients, 43 primary open-angle glaucoma (POAG) patients and 33 controls (candidates to cataract surgery). Methods: Twenty-seven inflammatory cytokines were analyzed in the tear and aqueous humor samples by the Bio-Plex Pro Human Cytokine 27-Plex Immunoassay kit: interleukin (IL)-1b, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12, IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony-stimulating factor, granulocyte-monocyte colony-stimulating factor, interferon-γ, interferon gamma-induced protein, monocyte chemo-attractant protein-1, macrophage inflammatory protein (MIP)-1a, MIP-1b, platelet-derived growth factor, regulated on activation normal T cell expressed and secreted, tumor necrosis factor-α and vascular endothelial growth factor.
 Main outcome measures: number of topical drugs, intraocular pressure, visual field mean defect and retinal nerve fiber layer global thickness measured by spectral-domain optical coherence tomography. Results: In the tear samples a statistically significant difference in Il-2, IL-4, IL-9, IL-12, IL-15 and FGF-basic between study groups was observed. In aqueous humor, a statistically significant difference of the following cytokines was observed: IL-5, IL-6, IL-12 and MCP-1. Il-4 and IL-15 differences correlate with the number of topical drugs, while IL-9 and IL-15 are associated with the visual field mean defect. Conclusion: Inflammation is strongly linked to glaucoma and some cytokines may be involved in the pathology of glaucoma.
UCM subjects