Lanthanide-doped calcium phosphate nanoparticles with high internal crystallinity and with a shell of DNA as fluorescent probes in cell experiments
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2007
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Royal Society of Chemistry
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Mondéjar SP, Kovtun A, Epple M. Lanthanide-doped calcium phosphate nanoparticles with high internal crystallinity and with a shell of DNA as fluorescent probes in cell experiments. J Mater Chem 2007;17:4153. https://doi.org/10.1039/b708258d
Abstract
Calcium phosphate nanoparticles were prepared by precipitation and stabilized as colloids by coating with DNA. They were doped with europium or terbium during this precipitation (about 2.5 wt%) and showed good fluorescence in the visible part of the spectrum. The fluorescence efficiency depended strongly on the internal crystallinity, i.e. amorphous nanoparticles of the same composition showed much weaker fluorescence. These particles are biocompatible and are easily taken up by cells (T-HUVEC), i.e. they can be used as fluorescing markers. Different colours can be realized by doping with different lanthanides.
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Se prepararon nanopartículas de fosfato de calcio por precipitación y se estabilizaron como coloides recubriéndolas con ADN. Se doparon con europio o terbio durante la precipitación (aproximadamente un 2,5% en peso) y mostraron una buena fluorescencia en la parte visible del espectro. La eficacia de la fluorescencia dependió en gran medida de la cristalinidad interna, es decir, las nanopartículas amorfas de la misma composición mostraban una fluorescencia mucho más débil. Estas partículas son biocompatibles, pueden fluorescer en diferentes colores dopándolas con diferentes lantánidos y son fácilmente internalizadas por las células (T-HUVEC), es decir, pueden ser utilizadas como marcadores fluorescentes.