Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’

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dc.contributor.authorRebollada Merino, Agustín Miguel
dc.contributor.authorUgarte Ruiz, María
dc.contributor.authorAlberto Gómez-Buendía
dc.contributor.authorBárcena Asensio, María Carmen
dc.contributor.authorGarcía Benzaquén, Nerea
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorRodríguez Bertos, Antonio Manuel
dc.date.accessioned2024-01-16T07:31:32Z
dc.date.available2024-01-16T07:31:32Z
dc.date.issued2022-10-17
dc.description.abstractAntimicrobial resistance demands the development of therapeutic alternatives such as prebiotics, probiotics, and nutraceuticals. The aim of this study was to assess the antimicrobial proprieties of the nutraceutical fermented defatted “alperujo”, derived from olive oil production, in a laying hen farm (n = 122,250) endemic with avian intestinal spirochetosis (Brachyspira spp.). Part of the batch (n = 1440) was divided into six groups of 240 hens each that included 80 or 108-week-old laying hens, supplemented with 0%, 2%, or 6% fermented defatted ‘alperujo’ for a month. At the end of the experiment, eight hens from each group were autopsied and cecal content was subjected to (i) Brachyspira culture and species identification by PCRs, and (ii) direct DNA extraction and Brachyspira qPCR. Furthermore, the ceca were processed for histopathology. Microbiological isolation revealed B. pilosicoli and B. hyodysenteriae co-infection in all groups. The 80-week-old hen group 2% supplemented showed a reduction in the cecal Brachyspira content (qPCR) compared with non-supplemented hens. Cecal histopathology showed a diffuse mild infiltration of lymphocytes, plasma cells, and heterophils; and hyperplasia of the gut-associated lymphoid tissue hyperplasia which decreased in severity in 80-week-old supplemented hens. The reduction in Brachyspira colonization and the severity of the lesions observed in supplemented hens highlights a potential protective function against avian intestinal spirochetosis.
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterios de Economía, Industria y Competetividad (RTC-2015-3621-2)
dc.description.sponsorshipComunidad de Madrid (S2013/ABI-2747)
dc.description.sponsorshipMinisterio de Ciencia e Innovación (RTI2018-098658-B-C22)
dc.description.sponsorshipMinisterio de Universidades (PRE2019-087439)
dc.description.statuspub
dc.identifier.citationRebollada-Merino, A.; Ugarte-Ruiz, M.; Gómez-Buendía, A.; Bárcena, C.; García, N.; Domínguez, L.; Rodríguez-Bertos, A. Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’. Fermentation 2022, 8, 550. https://doi.org/ 10.3390/fermentation8100550
dc.identifier.doi10.3390/fermentation8100550
dc.identifier.issn2311-5637
dc.identifier.officialurlhttps://www.mdpi.com/2311-5637/8/10/550
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93232
dc.journal.titleFermentation
dc.language.isoeng
dc.page.initial550
dc.publisherMDPI
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu579
dc.subject.cdu636.09
dc.subject.keywordBrachyspira
dc.subject.keywordIntestinal health
dc.subject.keywordLaying hen
dc.subject.keywordNutraceutical
dc.subject.keywordPoultry
dc.subject.ucmMicrobiología (Veterinaria)
dc.subject.ucmVeterinaria
dc.subject.unesco3109.05 Microbiología
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleCecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number8
dspace.entity.typePublication
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