Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food

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dc.contributor.authorGarcía Calvo, Eduardo Rafael
dc.contributor.authorGarcía García, Aina
dc.contributor.authorRodríguez, Santiago
dc.contributor.authorFarrais, Sergio
dc.contributor.authorMartín, Rosario
dc.contributor.authorGarcía, Teresa
dc.date.accessioned2023-06-22T12:48:05Z
dc.date.available2023-06-22T12:48:05Z
dc.date.issued2022-12-28
dc.description.abstractThe observed increase in the prevalence of gluten-related disorders has prompted the development of novel immunological systems for gluten detection in foodstuff. The innovation on these methods relies on the generation of new antibodies, which might alternatively be obtained by molecular evolution methods such as phage display. This work presents a novel approach for the generation of a Fab library by merging semi-synthetic heavy chains built-up from a pre-existent recombinant antibody fragment (dAb8E) with an immune light chain set derived from celiac donors. From the initial phage population (107 candidates) and after three rounds of selection and amplification, four different clones were isolated for further characterization. The phage Fab8E-4 presented the best features to be applied in an indirect ELISA for the detection of gluten in foods, resulting in improved specificity and sensitivity.
dc.description.departmentSección Dptal. de Nutrición y Ciencia de los Alimentos (Veterinaria)
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación
dc.description.sponsorshipComunidad de Madrid /FSE
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77384
dc.identifier.doi10.3390/foods12010149
dc.identifier.issn2304-8158
dc.identifier.officialurlhttps://doi.org/10.3390/foods12010149
dc.identifier.relatedurlhttps://www.mdpi.com/2304-8158/12/1/149
dc.identifier.urihttps://hdl.handle.net/20.500.14352/73196
dc.issue.number1
dc.journal.titleFoods
dc.language.isoeng
dc.page.initial149
dc.publisherMPDI
dc.relation.projectIDPID2021-122925OB-I00, PRE2018-08427
dc.relation.projectIDS2018/BAA-4574
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordGluten
dc.subject.keywordProlamins
dc.subject.keywordCeliac disease
dc.subject.keywordPhage display
dc.subject.keywordELISA
dc.subject.keywordImmune library
dc.subject.keywordSemi-synthetic library
dc.subject.ucmInmunología veterinaria
dc.subject.unesco3109.03 Inmunología
dc.titleConstruction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food
dc.typejournal article
dc.volume.number12
dspace.entity.typePublication
relation.isAuthorOfPublication5ad672ff-caca-43c2-87c7-289d7bae8aa1
relation.isAuthorOfPublication4c236a4b-c0dd-47b7-9bcd-4af6034724af
relation.isAuthorOfPublication.latestForDiscovery5ad672ff-caca-43c2-87c7-289d7bae8aa1
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