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Lichens of Parmelioid Clade as Promising Multitarget Neuroprotective Agents

dc.contributor.authorSieteiglesias Mansilla, Víctor
dc.contributor.authorGonzález Burgos, Elena María
dc.contributor.authorBermejo Bescos, María De La Paloma
dc.contributor.authorDulare Devi, Pradeep Divakar
dc.contributor.authorGómez-Serranillos Cuadrado, María Pilar
dc.date.accessioned2024-01-31T08:55:59Z
dc.date.available2024-01-31T08:55:59Z
dc.date.issued2019
dc.description.abstractNeurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease are multifactorial disorders which are increasing in incidence and prevalence over the world without existing effective therapies. The search for new multitarget compounds is the latter therapeutic strategy to address these pathological conditions. Lichens have an important and unknown therapeutic value attributed to their unique secondary metabolites. The aim of this study is to evaluate for the first time the in vitro neuroprotective activities and molecular mechanisms underlying methanol extracts of lichens of the parmelioid clade and to characterize major bioactive secondary metabolites responsible for their pharmacological actions. Of the 15 parmelioid lichen species, our results showed that Parmotrema perlatum and Hypotrachyna formosana methanol extracts exhibited high antioxidant activity as evidenced in ORAC, DPPH, and FRAP assays. Then, SH-SY5Y cells were pretreated with methanol extracts (24 h) followed by Fenton reagent exposure (2 h). Pretreatments with these two more antioxidant methanol lichen extracts increased cell viability, reduced intracellular ROS, prevented oxidative stress biomarkers accumulation, and upregulated antioxidant enzyme (CAT, SOD, GR, and GPx) activity compared to Fenton reagent cells. The neuroprotective activity was much higher for H. formosana than for P. perlatum, even equal to or higher than Trolox (reference compound). Moreover, H. formosana extracts inhibited both AChE and BuChE activities in a concentration dependent manner, and P. perlatum only showed concentration dependent activity against AChE. Finally, chemical composition analysis using TLC and HPLC methods revealed that physodic acid, lividic acid, and lichexanthone are major secondary metabolites in H. formosana and stictic acid and constictic acid are in P. perlatum. These results demonstrated that P. perlatum and, specially, H. formosana are promising multitargeted neuroprotective agents due to their antioxidant and AChE and BuChE inhibition activities.eng
dc.description.departmentDepto. de Farmacología, Farmacognosia y Botánica
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationSieteiglesias, Víctor, et al. «Lichens of Parmelioid Clade as Promising Multitarget Neuroprotective Agents». Chemical Research in Toxicology, vol. 32, n.o 6, junio de 2019, pp. 1165-77. DOI.org (Crossref), https://doi.org/10.1021/acs.chemrestox.9b00010.
dc.identifier.doi10.1021/acs.chemrestox.9b00010
dc.identifier.essn1520-5010
dc.identifier.issn0893-228X
dc.identifier.officialurlhttps://doi.org/10.1021/acs.chemrestox.9b00010
dc.identifier.relatedurlhttps://pubs.acs.org/doi/10.1021/acs.chemrestox.9b00010
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96888
dc.language.isoeng
dc.rights.accessRightsrestricted access
dc.subject.keywordAlcohols
dc.subject.keywordAntioxidants
dc.subject.keywordAromatic compounds
dc.subject.keywordPeptides and proteins
dc.subject.keywordReagents
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco32 Ciencias Médicas
dc.titleLichens of Parmelioid Clade as Promising Multitarget Neuroprotective Agents
dc.typejournal article
dspace.entity.typePublication
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relation.isAuthorOfPublication69510424-a309-4eeb-b62d-df87d56e477a
relation.isAuthorOfPublicatione522c572-7726-486a-9247-401ae028e175
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relation.isAuthorOfPublication.latestForDiscoverya74693cd-ed68-4c7a-9c58-0b391d1bfdd2

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