Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement
dc.contributor.author | Martín Escudero, M. Del Pilar | |
dc.contributor.author | Muñoz Guerra, Jesus A. | |
dc.contributor.author | García Tenorio, Soledad Vargas | |
dc.contributor.author | Serrano Garde, Ester | |
dc.contributor.author | Soldevilla Navarro, Ana Belén | |
dc.contributor.author | Cortes Carrillo, Nuria | |
dc.contributor.author | Galindo Canales, Mercedes | |
dc.contributor.author | del Prado, Nayade | |
dc.contributor.author | Fuentes Ferrer, Manuel | |
dc.contributor.author | Fernández Pérez, Cristina | |
dc.contributor.author | Behnisch, Peter Alexander | |
dc.contributor.author | Brouwer, Abraham | |
dc.date.accessioned | 2023-06-17T08:32:21Z | |
dc.date.available | 2023-06-17T08:32:21Z | |
dc.date.issued | 2021-03-26 | |
dc.description | The study was funded by grants awarded by the Spanish Ministry of Science and Innovation (DEP2016–78559-R; sub-program DEPO; DEP2012-40156) for competitive investigation. | |
dc.description.abstract | The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX® bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type. | |
dc.description.faculty | Fac. de Medicina | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Ciencia e Innovación (España) | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/77475 | |
dc.identifier.doi | 10.3390/chemosensors9040062 | |
dc.identifier.issn | 2227-9040 | |
dc.identifier.officialurl | https://doi.org/10.3390/chemosensors9040062 | |
dc.identifier.relatedurl | https://www.mdpi.com/2227-9040/9/4/62 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/7394 | |
dc.issue.number | 4 | |
dc.journal.title | Chemosensors | |
dc.language.iso | eng | |
dc.page.initial | 62 | |
dc.publisher | MDPI | |
dc.relation.projectID | DEP2016–78559-R; sub-program DEPO; DEP2012-40156 | |
dc.rights | Atribución 3.0 España | |
dc.rights.accessRights | open access | |
dc.rights.uri | https://creativecommons.org/licenses/by/3.0/es/ | |
dc.subject.cdu | 61:796 | |
dc.subject.keyword | Doping | |
dc.subject.keyword | UGT2B17 genotype | |
dc.subject.keyword | Steroid profile | |
dc.subject.keyword | Testosterone administration | |
dc.subject.keyword | Sport | |
dc.subject.keyword | Athlete biological passport | |
dc.subject.keyword | AR CALUX® | |
dc.subject.keyword | Androgenic activity | |
dc.subject.ucm | Medicina | |
dc.subject.ucm | Medicina del deporte | |
dc.subject.unesco | 32 Ciencias Médicas | |
dc.title | Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement | |
dc.type | journal article | |
dc.volume.number | 9 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 773a796c-300d-4a3e-9e16-0e3880112a01 | |
relation.isAuthorOfPublication | e3b551e4-0127-4a0e-ad9e-2232be67b78b | |
relation.isAuthorOfPublication.latestForDiscovery | e3b551e4-0127-4a0e-ad9e-2232be67b78b |
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