Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement

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dc.contributor.authorMartín Escudero, M. Del Pilar
dc.contributor.authorMuñoz Guerra, Jesus A.
dc.contributor.authorGarcía Tenorio, Soledad Vargas
dc.contributor.authorSerrano Garde, Ester
dc.contributor.authorSoldevilla Navarro, Ana Belén
dc.contributor.authorCortes Carrillo, Nuria
dc.contributor.authorGalindo Canales, Mercedes
dc.contributor.authordel Prado, Nayade
dc.contributor.authorFuentes Ferrer, Manuel
dc.contributor.authorFernández Pérez, Cristina
dc.contributor.authorBehnisch, Peter Alexander
dc.contributor.authorBrouwer, Abraham
dc.date.accessioned2023-06-17T08:32:21Z
dc.date.available2023-06-17T08:32:21Z
dc.date.issued2021-03-26
dc.descriptionThe study was funded by grants awarded by the Spanish Ministry of Science and Innovation (DEP2016–78559-R; sub-program DEPO; DEP2012-40156) for competitive investigation.
dc.description.abstractThe anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX® bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type.
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77475
dc.identifier.doi10.3390/chemosensors9040062
dc.identifier.issn2227-9040
dc.identifier.officialurlhttps://doi.org/10.3390/chemosensors9040062
dc.identifier.relatedurlhttps://www.mdpi.com/2227-9040/9/4/62
dc.identifier.urihttps://hdl.handle.net/20.500.14352/7394
dc.issue.number4
dc.journal.titleChemosensors
dc.language.isoeng
dc.page.initial62
dc.publisherMDPI
dc.relation.projectIDDEP2016–78559-R; sub-program DEPO; DEP2012-40156
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu61:796
dc.subject.keywordDoping
dc.subject.keywordUGT2B17 genotype
dc.subject.keywordSteroid profile
dc.subject.keywordTestosterone administration
dc.subject.keywordSport
dc.subject.keywordAthlete biological passport
dc.subject.keywordAR CALUX®
dc.subject.keywordAndrogenic activity
dc.subject.ucmMedicina
dc.subject.ucmMedicina del deporte
dc.subject.unesco32 Ciencias Médicas
dc.titleBioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement
dc.typejournal article
dc.volume.number9
dspace.entity.typePublication
relation.isAuthorOfPublication773a796c-300d-4a3e-9e16-0e3880112a01
relation.isAuthorOfPublicatione3b551e4-0127-4a0e-ad9e-2232be67b78b
relation.isAuthorOfPublication.latestForDiscoverye3b551e4-0127-4a0e-ad9e-2232be67b78b
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