Highly fluorescent magnetic nanobeads with a remarkable stokes shift as labels for enhanced detection in immunoassays

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dc.contributor.authorSalis, Francesca
dc.contributor.authorDescalzo López, Ana Belén
dc.contributor.authorBenito Peña, María Elena
dc.contributor.authorMoreno Bondi, María Cruz
dc.contributor.authorOrellana Moraleda, Guillermo
dc.date.accessioned2024-01-16T11:04:11Z
dc.date.available2024-01-16T11:04:11Z
dc.date.issued2018-05-17
dc.description.abstractFluorescence immunoassays are popular for achieving high sensitivity, but they display limitations in biological samples due to strong absorption of light, background fluorescence from matrix components, or light scattering by the biomacromolecules. A powerful strategy to overcome these problems is introduced here by using fluorescent magnetic nanobeads doped with two boron-dipyrromethane dyes displaying intense emission in the visible and near-infrared regions, respectively. Careful matching of the emission and absorption features of the dopants leads to a virtual Stokes shift larger than 150 nm achieved by an intraparticle Förster resonance energy transfer (FRET) process between the donor and the acceptor dyes. Additionally, the magnetic properties of the fluorescent beads allow preconcentration of the sample. To illustrate the usefulness of this approach to increase the sensitivity of fluorescence immunoassays, the novel nanoparticles are employed as labels for quantification of the widely used Tacrolimus (FK506) immunosuppressive drug. The FRET-based competitive inhibition immunoassay yields a limit of detection (LOD) of 0.08 ng/mL, with a dynamic range (DR) of 0.15–2.0 ng/mL, compared to a LOD of 2.7 ng/mL and a DR between 4.1 and 130 ng/mL for the immunoassay carried out with direct excitation of the acceptor dye.
dc.description.departmentDepto. de Química Orgánica
dc.description.departmentDepto. de Química Analítica
dc.description.facultyCAI Ciencias de la Tierra y Arqueometría
dc.description.refereedTRUE
dc.description.sponsorshipEuropean Funds for Regional Development (FEDER)
dc.description.sponsorshipSpanish Ministry of Economy and Competitiveness
dc.description.statuspub
dc.identifier.doi10.1002/smll.201703810
dc.identifier.issn1613-6810
dc.identifier.issn1613-6829
dc.identifier.officialurlhttps://doi.org/10.1002/smll.201703810
dc.identifier.relatedurlhttps://onlinelibrary.wiley.com/journal/16136829
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93321
dc.issue.number20
dc.journal.titleSmall
dc.language.isoeng
dc.page.final10
dc.page.initial1
dc.publisherWiley
dc.relation.projectIDEuropean Union NMP Research Program under contract no. 318372 (“NANODEM”)
dc.relation.projectIDCTQ2015-69278C2-R/AIE
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordFluorescent magnetic nanoparticles
dc.subject.keywordFRET
dc.subject.keywordBODIPY
dc.subject.keywordTacrolimus
dc.subject.keywordimmunoassay
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco23 Química
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco33 Ciencias Tecnológicas
dc.titleHighly fluorescent magnetic nanobeads with a remarkable stokes shift as labels for enhanced detection in immunoassays
dc.typejournal article
dc.type.hasVersionSMUR
dc.volume.number14
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscoveryebb3e2fd-e5d5-4a84-9ce0-c9ea12eb2a85
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