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Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD

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dc.contributor.authorTruchado Martín, Daniel Alejandro
dc.contributor.authorMedrano Arranz, Carlos
dc.contributor.authorRincón, Sara
dc.contributor.authorZurita, Lucía
dc.contributor.authorPonz, Fernando
dc.date.accessioned2024-04-17T17:57:36Z
dc.date.available2024-04-17T17:57:36Z
dc.date.issued2024-04-07
dc.description.abstractNanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2. Our findings show the high potential of TuMV nanoparticles in the fields of diagnostics and immunodetection, being especially attractive for the development of novel antibody sensing devices.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Universidades (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.identifier.doi10.1016/j.diagmicrobio.2024.116298
dc.identifier.issn0732-8893
dc.identifier.officialurlhttps://doi.org/10.1016/j.diagmicrobio.2024.116298
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103201
dc.issue.number3
dc.journal.titleDiagnostic Microbiology & Infectious Disease
dc.language.isoeng
dc.page.final7
dc.page.initial1
dc.publisherElsevier
dc.relation.projectIDCOV20/00114
dc.relation.projectIDP2018/BAA-4574
dc.relation.projectIDCT31/21
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu577.27
dc.subject.cdu579.61
dc.subject.keywordTurnip mosaic virus
dc.subject.keywordSARS-CoV-2
dc.subject.keywordVLP
dc.subject.keywordAntibody sensing
dc.subject.keywordSaliva
dc.subject.keywordIgA
dc.subject.ucmMicrobiología (Biología)
dc.subject.ucmInmunología
dc.subject.ucmBiotecnología
dc.subject.unesco2414 Microbiología
dc.subject.unesco2420 Virología
dc.subject.unesco2412 Inmunología
dc.titleAntigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number109
dspace.entity.typePublication
relation.isAuthorOfPublication9c46c9d4-1201-4d42-b751-f62109a8154a
relation.isAuthorOfPublication.latestForDiscovery9c46c9d4-1201-4d42-b751-f62109a8154a

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