Publication:
Electrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes

dc.contributor.authorSánchez Tirado, E.
dc.contributor.authorSalvo, C.
dc.contributor.authorGónzalez Cortés, A
dc.contributor.authorYáñez Sedeño, Paloma
dc.contributor.authorLanga, F.
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-17T22:41:53Z
dc.date.available2023-06-17T22:41:53Z
dc.date.issued2017-03
dc.description.abstractDual screen-printed carbon electrodes modified with 4-carboxyphenyl-functionalized double-walled carbon nanotubes (HOOC-Phe-DWCNTs/SPCEs) have been used as scaffolds for the preparation of electrochemical immunosensors for the simultaneous determination of the cytokines Interleukin-1b (IL-1b) and factor necrosis tumor a (TNF-a). IL-1b. Capture antibodies were immobilized onto HOOC-PheDWCNTs/SPCEs in an oriented form making using the commercial polymeric coating Mix&Go™. Sandwich type immunoassays with amperometric signal amplification through the use of poly-HRPstreptavidin conjugates and H2O2 as HRP substrate and hydroquinone as redox mediator were implemented. Upon optimization of the experimental variables affecting the immunosensor performance, the dual immunosensor allows ranges of linearity extending between 0.5 and 100 pg/mL and from 1 to 200 pg/mL for IL-1b and TNF-a, respectively, these ranges being adequate for the determination of the cytokines in clinical samples. The achieved limits of detection were 0.38 pg/mL (IL-1b) and 0.85 pg/mL (TNF-a). In addition, the dual immunosensor exhibits excellent reproducibility of the measurements, storage stability of the anti-IL-Phe-DWCNTs/SPCE and anti-TNF-Phe-DWCNTs/SPCE conjugates, and selectivity as well as negligible cross-talking. The dual immunosensor was applied to the simultaneous determination of IL-1b and TNF-a in human serum spiked at clinically relevant concentration levels and in real saliva samples.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/55184
dc.identifier.doi10.1016/j.aca.2016.12.034
dc.identifier.issn0003-2670
dc.identifier.officialurlhttps://www.ncbi.nlm.nih.gov/pubmed/28159106
dc.identifier.urihttps://hdl.handle.net/20.500.14352/18769
dc.issue.number959
dc.journal.titleAnalytica Chimica Acta
dc.language.isoeng
dc.page.final73
dc.page.initial66
dc.publisherElsevier
dc.relation.projectID(CTQ2015-70023-R ; CTQ2015- 71955-REDT)
dc.relation.projectIDNANOAVANSENS (S2013/MT-3029)
dc.rights.accessRightsopen access
dc.subject.cdu543
dc.subject.keywordInterleukin-1 beta Tumor necrosis factor-alpha Electrochemical immunosensor Multiplex Double-walled carbon nanotubes Saliva
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical immunosensor for simultaneous determination of interleukin-1 beta and tumor necrosis factor alpha in serum and saliva using dual screen printed electrodes modified with functionalized doubleewalled carbon nanotubes
dc.typejournal article
dc.volume.number22
dspace.entity.typePublication
relation.isAuthorOfPublication8808f99c-5f56-4562-839a-517626c76dad
relation.isAuthorOfPublication.latestForDiscovery8808f99c-5f56-4562-839a-517626c76dad
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