Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli

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dc.contributor.authorDíaz Del Toro, Silvia
dc.contributor.authorAguilera, Ángeles
dc.contributor.authorGonzález de Figueras, Carolina
dc.contributor.authorDe Francisco Martínez, Patricia
dc.contributor.authorOlsson, Sanna
dc.contributor.authorPuente-Sánchez, Fernando
dc.contributor.authorGonzález-Pastor, José Eduardo
dc.date.accessioned2024-04-12T09:58:46Z
dc.date.available2024-04-12T09:58:46Z
dc.date.issued2022-06-23
dc.description.abstractPhytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MCIN)/Agencia estatal de Investigación
dc.description.sponsorshipFondo Europeo de Desarrollo Regional (FEDER)
dc.description.statuspub
dc.identifier.doi10.3390/ijerph19137692
dc.identifier.essn2008-2304
dc.identifier.officialurlhttps://www.mdpi.com/1660-4601/19/13/7692
dc.identifier.urihttps://hdl.handle.net/20.500.14352/103047
dc.issue.number13
dc.journal.titleInternational journal of environmental research
dc.language.isoeng
dc.page.final21
dc.page.initial1
dc.publisherMDPI
dc.relation.projectID(RTI2018-094867-B-I00, PID2019-104205GB-C22, and PGC2018-096956-B-C42)
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu579.25
dc.subject.keywordPhytochelatins
dc.subject.keywordHeavy metal resistance
dc.subject.keywordStress response
dc.subject.keywordOxidative stress
dc.subject.keywordBioaccumulation
dc.subject.ucmMicrobiología (Biología)
dc.subject.ucmGenética
dc.subject.unesco3108.01 Bacterias
dc.subject.unesco2409 Genética
dc.titleHeterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number19
dspace.entity.typePublication
relation.isAuthorOfPublication6051af90-2d20-4bff-baec-253f6c366f39
relation.isAuthorOfPublication469a6187-001d-4d49-a795-9072ff43f702
relation.isAuthorOfPublication.latestForDiscovery6051af90-2d20-4bff-baec-253f6c366f39
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