Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
dc.contributor.author | Díaz Del Toro, Silvia | |
dc.contributor.author | Aguilera, Ángeles | |
dc.contributor.author | González de Figueras, Carolina | |
dc.contributor.author | De Francisco Martínez, Patricia | |
dc.contributor.author | Olsson, Sanna | |
dc.contributor.author | Puente-Sánchez, Fernando | |
dc.contributor.author | González-Pastor, José Eduardo | |
dc.date.accessioned | 2024-04-12T09:58:46Z | |
dc.date.available | 2024-04-12T09:58:46Z | |
dc.date.issued | 2022-06-23 | |
dc.description.abstract | Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation. | |
dc.description.department | Depto. de Genética, Fisiología y Microbiología | |
dc.description.faculty | Fac. de Ciencias Biológicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Ciencia e Innovación (MCIN)/Agencia estatal de Investigación | |
dc.description.sponsorship | Fondo Europeo de Desarrollo Regional (FEDER) | |
dc.description.status | pub | |
dc.identifier.doi | 10.3390/ijerph19137692 | |
dc.identifier.essn | 2008-2304 | |
dc.identifier.officialurl | https://www.mdpi.com/1660-4601/19/13/7692 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/103047 | |
dc.issue.number | 13 | |
dc.journal.title | International journal of environmental research | |
dc.language.iso | eng | |
dc.page.final | 21 | |
dc.page.initial | 1 | |
dc.publisher | MDPI | |
dc.relation.projectID | (RTI2018-094867-B-I00, PID2019-104205GB-C22, and PGC2018-096956-B-C42) | |
dc.rights | Attribution 4.0 International | en |
dc.rights.accessRights | open access | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.cdu | 579.25 | |
dc.subject.keyword | Phytochelatins | |
dc.subject.keyword | Heavy metal resistance | |
dc.subject.keyword | Stress response | |
dc.subject.keyword | Oxidative stress | |
dc.subject.keyword | Bioaccumulation | |
dc.subject.ucm | Microbiología (Biología) | |
dc.subject.ucm | Genética | |
dc.subject.unesco | 3108.01 Bacterias | |
dc.subject.unesco | 2409 Genética | |
dc.title | Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli | |
dc.type | journal article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 19 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 6051af90-2d20-4bff-baec-253f6c366f39 | |
relation.isAuthorOfPublication | 469a6187-001d-4d49-a795-9072ff43f702 | |
relation.isAuthorOfPublication.latestForDiscovery | 6051af90-2d20-4bff-baec-253f6c366f39 |
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