Transcriptomic Profile of Canine DH82 Macrophages Infected by Leishmania infantum Promastigotes with Different Virulence Behavior

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dc.contributor.authorMas Zubiri, Alicia
dc.contributor.authorMartínez Rodrigo, Abel
dc.contributor.authorCarrión Herrero, Francisco Javier
dc.contributor.authorOrden Gutiérrez, José Antonio
dc.contributor.authorAlzate, Juan F.
dc.contributor.authorDomínguez Bernal, Gustavo Ramón
dc.contributor.authorHorcajo Iglesias, María Del Pilar
dc.date.accessioned2023-06-22T12:49:37Z
dc.date.available2023-06-22T12:49:37Z
dc.date.issued2022-01-27
dc.description.abstractZoonotic visceral leishmaniosis caused by Leishmania infantum is an endemic disease in the Mediterranean Basin affecting mainly humans and dogs, the main reservoir. The leishmaniosis outbreak declared in the Community of Madrid (Spain) led to a significant increase in human disease incidence without enhancing canine leishmaniosis prevalence, suggesting a better adaptation of the outbreak’s isolates by other host species. One of the isolates obtained in the focus, IPER/ES/2012/BOS1FL1 (BOS1FL1), has previously demonstrated a different phenotype than the reference strain MCAN/ES/1996/BCN150 (BCN150), characterized by a lower infectivity when interacting with canine macrophages. Nevertheless, not enough changes in the cell defensive response were found to support their different behavior. Thus, we decided to investigate the molecular mechanisms involved in the interaction of both parasites with DH82 canine macrophages by studying their transcriptomic profiles developed after infection using RNA sequencing. The results showed a common regulation induced by both parasites in the phosphoinositide-3-kinase–protein kinase B/Akt and NOD-like receptor signaling pathways. However, other pathways, such as phagocytosis and signal transduction, including tumor necrosis factor, mitogen-activated kinases and nuclear factor-κB, were only regulated after infection with BOS1FL1. These differences could contribute to the reduced infection ability of the outbreak isolates in canine cells. Our results open a new avenue to investigate the true role of adaptation of L. infantum isolates in their interaction with their different hosts.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipUCM-Santander
dc.description.sponsorshipCommunity of Madrid PLATESA2-CM
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77414
dc.identifier.doi10.3390/ijms23031466
dc.identifier.issn1422-0067
dc.identifier.officialurlhttps://doi.org/10.3390/ijms23031466
dc.identifier.relatedurlhttps://www.mdpi.com/1422-0067/23/3/1466
dc.identifier.urihttps://hdl.handle.net/20.500.14352/73226
dc.issue.number3
dc.journal.titleInternational Journal of Molecular Sciences
dc.language.isoeng
dc.page.initial1466
dc.publisherMPDI
dc.relation.projectIDPR75/18-21558
dc.relation.projectIDS2018/BAA-4370, PID2019-106487RB-I00/AEI/10.13039/501100011033
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordLeishmania infantum
dc.subject.keywordvirulence
dc.subject.keywordcanine macrophage
dc.subject.keywordRNA-seq
dc.subject.keywordDH82 cells
dc.subject.ucmSanidad animal
dc.titleTranscriptomic Profile of Canine DH82 Macrophages Infected by Leishmania infantum Promastigotes with Different Virulence Behavior
dc.typejournal article
dc.volume.number23
dspace.entity.typePublication
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