Characterization of plasmids in a human clinical strain of Lactococcus garvieae

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dc.contributor.authorAguado Urda, Mónica
dc.contributor.authorGibello Prieto, Alicia
dc.contributor.authorBlanco Gutiérrez, María Del Mar
dc.contributor.authorLópez Campos, Guillermo H
dc.contributor.authorCutuli Simón, María Teresa
dc.contributor.authorFernández-Garayzábal Fernández, José Francisco
dc.date.accessioned2023-06-20T04:19:21Z
dc.date.available2023-06-20T04:19:21Z
dc.date.issued2012
dc.description.abstractThe present work describes the molecular characterization of five circular plasmids found in the human clinical strain Lactococcus garvieae 21881. The plasmids were designated pGL1-pGL5, with molecular sizes of 4,536 bp, 4,572 bp, 12,948 bp, 14,006 bp and 68,798 bp, respectively. Based on detailed sequence analysis, some of these plasmids appear to be mosaics composed of DNA obtained by modular exchange between different species of lactic acid bacteria. Based on sequence data and the derived presence of certain genes and proteins, the plasmid pGL2 appears to replicate via a rolling-circle mechanism, while the other four plasmids appear to belong to the group of lactococcal theta-type replicons. The plasmids pGL1, pGL2 and pGL5 encode putative proteins related with bacteriocin synthesis and bacteriocin secretion and immunity. The plasmid pGL5 harbors genes (txn, orf5 and orf25) encoding proteins that could be considered putative virulence factors. The gene txn encodes a protein with an enzymatic domain corresponding to the family actin-ADP-ribosyltransferases toxins, which are known to play a key role in pathogenesis of a variety of bacterial pathogens. The genes orf5 and orf25 encode two putative surface proteins containing the cell wall-sorting motif LPXTG, with mucin-binding and collagen-binding protein domains, respectively. These proteins could be involved in the adherence of L. garvieae to mucus from the intestine, facilitating further interaction with intestinal epithelial cells and to collagenous tissues such as the collagen-rich heart valves. To our knowledge, this is the first report on the characterization of plasmids in a human clinical strain of this pathogen.
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. FP7
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39666
dc.identifier.doi10.1371/journal.pone.0040119
dc.identifier.issn1932-6203
dc.identifier.officialurlhttp://dx.doi.org/10.1371/journal.pone.0040119
dc.identifier.urihttps://hdl.handle.net/20.500.14352/45166
dc.issue.number6
dc.journal.titlePLoS ONE
dc.language.isoeng
dc.page.initiale40119
dc.publisherPublic Library Science
dc.relation.projectIDINBIOMEDvision (270107)
dc.relation.projectIDAGL2009-12447
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleCharacterization of plasmids in a human clinical strain of Lactococcus garvieae
dc.typejournal article
dc.volume.number7
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscoverya3fc3ed4-b6be-4c56-a6fd-e315e8b4eb85
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