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Susceptibilities of an irreversible Michaelis-Menten enzyme

dc.contributor.authorSánchez Torralba, Antonio
dc.contributor.authorRodríguez, Yoel
dc.date.accessioned2025-01-29T16:01:03Z
dc.date.available2025-01-29T16:01:03Z
dc.date.issued2006
dc.description.abstractThe nonlinear response of the simplest irreversible Michaelis-Menten enzyme is considered. In the context of metabolic networks, i.e. in vivo, the enzyme is subject to sustained, frequently time-dependent, input fluxes that keep the system out of equilibrium. The connection between the fluxes and the response is investigated by means of a new sensitivity analysis. The kinetics of the enzyme is simple enough to allow for the computations to be carried out analytically. In particular, a set of sensitivities of the response with respect to the substrate influx, the susceptibilities, is derived. The susceptibilities are multivariate functions and thus are suitable for predicting complete progress curves of several variables of biochemical interest, namely, rates and concentrations. This is shown by means of an example. The relationship between the susceptibilities and the stoichiometry of the reaction is also taken into account. Moreover, all the required information comes from decay experiments of initial concentrations, which are common in enzymological setups.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipDGICYT, Ministerio de Ciencia y Tecnologı́a
dc.description.statuspub
dc.identifier.citationTorralba, A.S., Rodríguez, Y. Susceptibilities of an Irreversible Michaelis – Menten Enzyme. Bull. Math. Biol. 68, 1335–1353 (2006)
dc.identifier.doi10.1007/s11538-006-9111-y
dc.identifier.essn1522-9602
dc.identifier.issn0092-8240
dc.identifier.officialurlhttps://doi.org/10.1007/s11538-006-9111-y
dc.identifier.pmid17149820
dc.identifier.urihttps://hdl.handle.net/20.500.14352/116981
dc.issue.number6
dc.journal.titleBulletin of Mathematical Biology
dc.language.isoeng
dc.page.final1353
dc.page.initial1335
dc.publisherSpringer Nature Link
dc.relation.projectIDBMC2000-0554
dc.rights.accessRightsrestricted access
dc.subject.cdu54
dc.subject.keywordEnzyme kinetics
dc.subject.keywordTransients
dc.subject.keywordNon-equilibrium response
dc.subject.keywordSteady-state response
dc.subject.keywordLarge excitations
dc.subject.ucmBiología molecular (Química)
dc.subject.ucmQuímica física (Química)
dc.subject.unesco2302 Bioquímica
dc.subject.unesco2302.09 Enzimología
dc.subject.unesco2307 Química Física
dc.titleSusceptibilities of an irreversible Michaelis-Menten enzyme
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number68
dspace.entity.typePublication
relation.isAuthorOfPublicationd183ea55-d42c-4655-8408-1f009cb16cdd
relation.isAuthorOfPublication.latestForDiscoveryd183ea55-d42c-4655-8408-1f009cb16cdd

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