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Identification and characterisation of the proteins bound by specific phage‐displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts

dc.contributor.authorCruz. Silvia de la
dc.contributor.authorMadrid García, Raquel
dc.contributor.authorGarcía García, Aina
dc.contributor.authorAlcocer, Marcos
dc.contributor.authorMartín De Santos, María Del Rosario
dc.contributor.authorGonzález Alonso, María Isabel
dc.contributor.authorGarcía Lacarra, Teresa
dc.date.accessioned2024-01-20T16:41:16Z
dc.date.available2024-01-20T16:41:16Z
dc.date.issued2017
dc.description.abstractBACKGROUND: Almonds and Brazil nuts are widely consumed allergenic nuts whose presence must be declared according to food labelling regulations. Their detection in food products has been recently achieved by ELISA methods with recombinant antibodies (scFv) isolated against complete Brazil nut and almond protein extracts. The screening of phage-scFv libraries against complete protein extracts confers a series of advantages over the use of purified proteins, as recombinant proteins might alter their native folding. However, using this strategy, the nature of the target detected by phage-displayed antibodies remains unknown, and requires further research to identify whether they are nut allergens or other molecules present in the extract, but not related to their allergenic potential. RESULTS: Electrophoretic, chromatographic, immunological and spectrometric techniques revealed that the Brazil nut (BE95) and almond (PD1F6 and PD2C9) specific phage-scFvs detected conformational epitopes of the Brazil nut and almond 11S globulins, recognised by WHO/IUIS as Ber e 2 and Pru du 6 major allergens. Circular dichroism data indicated that severe heat treatment would entail loss of epitope structure, disabling scFv for target detection. CONCLUSIONS: The presence of important Brazil nut and almond allergens (Ber e 2 and Pru du 6) in foodstuffs can be determined by using phage-display antibodies BE95, PD1F6 and PD2C9 as affinity probes in ELISA.
dc.description.departmentDepto. de Nutrición y Ciencia de los Alimentos
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.statuspub
dc.identifier.citationde la Cruz, S., Madrid, R., García-García, A., Alcocer, M., Martín, R., González, I., & García, T. (2018). Identification and characterisation of the proteins bound by specific phage-displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts. Journal of the science of food and agriculture, 98(5), 1685–1695. https://doi.org/10.1002/jsfa.8640
dc.identifier.doi10.1002/jsfa.8640
dc.identifier.essn1097-0010
dc.identifier.issn0022-5142
dc.identifier.officialurlhttps://www.doi.org/10.1002/jsfa.8640
dc.identifier.pmid28846130
dc.identifier.relatedurlhttps://onlinelibrary.wiley.com/doi/10.1002/jsfa.8640
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94201
dc.issue.number5
dc.journal.titleJournal of the Science of Food and Agriculture
dc.language.isoeng
dc.page.final1695
dc.page.initial1685
dc.publisherWiley
dc.relation.projectIDAGL 2013-48018-R
dc.relation.projectIDS2013/AB12747
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keyword11S globulin
dc.subject.keywordBrazil nut
dc.subject.keywordAlmond
dc.subject.keywordAmandin
dc.subject.keywordScFv
dc.subject.ucmVeterinaria
dc.subject.unesco31 Ciencias Agrarias
dc.titleIdentification and characterisation of the proteins bound by specific phage‐displayed recombinant antibodies (scFv) obtained against Brazil nut and almond extracts
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number98
dspace.entity.typePublication
relation.isAuthorOfPublication92f11fce-d91b-47eb-8e14-bd4ce6c79cf6
relation.isAuthorOfPublication4c236a4b-c0dd-47b7-9bcd-4af6034724af
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relation.isAuthorOfPublication.latestForDiscovery92f11fce-d91b-47eb-8e14-bd4ce6c79cf6

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