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Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

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dc.contributor.authorBlanco, María José
dc.contributor.authorLearte, Ana
dc.contributor.authorMarchena, Miguel
dc.contributor.authorMuñoz-Sáez, Emma
dc.contributor.authorCid, María Antonia
dc.contributor.authorRodríguez-Martín, Iván
dc.contributor.authorSánchez-Camacho Blázquez, Cristina
dc.date.accessioned2024-02-05T11:36:08Z
dc.date.available2024-02-05T11:36:08Z
dc.date.issued2018
dc.description.abstractHere we describe the standard protocol for the detection of β-galactosidase activity in early whole mouse embryos and the method for paraffin sectioning and counterstaining. This is an easy and quick procedure to monitor gene expression during development that can also be applied to tissue sections, organs or cultured cells.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipUniversidad Europea de Madrid
dc.description.statuspub
dc.identifier.citationBlanco, M. J., Learte, A. I., Marchena, M. A., Muñoz-Sáez, E., Cid, M. A., Rodríguez-Martín, I., Sánchez-Camacho, C. Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos. J. Vis. Exp. (136), e57785, doi:10.3791/57785 (2018).
dc.identifier.doi10.3791/57785
dc.identifier.essn1940-087X
dc.identifier.officialurlhttps://doi.org/10.3791/57785
dc.identifier.urihttps://hdl.handle.net/20.500.14352/98845
dc.journal.titleJournal of Visualized Experiments
dc.language.isoeng
dc.publisherMyJove Corporation
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.ucmCiencias
dc.subject.unesco24 Ciencias de la Vida
dc.titleTracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number136
dspace.entity.typePublication
relation.isAuthorOfPublication2ce1597c-df9d-46cd-b799-7b421378ee34
relation.isAuthorOfPublication.latestForDiscovery2ce1597c-df9d-46cd-b799-7b421378ee34

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