Effects of glucose and insulin on glucokinase activity in rat hypothalamus

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dc.contributor.authorSanz Miguel, María Del Carmen
dc.contributor.authorRoncero Rincón, Isabel
dc.contributor.authorVázquez Pérez, Patricia
dc.contributor.authorNavas Hernández, María Ángeles
dc.contributor.authorBlázquez Fernández, Enrique
dc.date.accessioned2024-01-29T15:33:00Z
dc.date.available2024-01-29T15:33:00Z
dc.date.issued2007
dc.description.abstractIn an attempt to study the role of glucokinase (GK) and the effects of glucose and peptides on GK gene expression and on the activity of this enzyme in the hypothalamus, we used two kinds of biological models: hypothalamic GT1-7 cells and rat hypothalamic slices. The expression of the GK gene in GT1-7 cells was reduced by insulin (INS) and was not modified by different glucose concentrations, while GK enzyme activities were significantly reduced by the different peptides. Interestingly, a distinctive pattern of GK activities between the ventromedial hypothalamus (VMH) and lateral hypothalamus (LH) were found, with higher enzyme activities in the VMH as the glucose concentrations rose, while LH enzyme activities decreased at 2.8 and 20 mM glucose, the latter effect being prevented by incubation with INS. These effects were produced only by d-glucose and the modifications found were due to GK, but not to other hexokinases. In addition, GK activities in the VMH and the LH were reduced by glucagon-like peptide 1, leptin, orexin B, INS, and neuropeptide Y (NPY), but this effect was only statistically significant for NPY in LH. Our results indicate that the effects of both glucose and peptides occur on GK enzyme activities rather than on GK gene transcription. Moreover, the effects of glucose and INS on GK activity suggest that in the brain GK behaves in a manner opposite to that in the liver, which might facilitate its role in glucose sensing. Finally, hypothalamic slices seem to offer a good physiological model to discriminate the effects between different areas.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationSanz C, Roncero I, Vázquez P, Navas MA, Blázquez E. Effects of glucose and insulin on glucokinase activity in rat hypothalamus. J Endocrinol. 2007;193(2):259-267. doi:10.1677/JOE-06-0146
dc.identifier.doi10.1677/joe-06-0146
dc.identifier.issn0022-0795
dc.identifier.issn1479-6805
dc.identifier.officialurlhttps://doi.org/10.1677/JOE-06-0146
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96175
dc.issue.number2
dc.journal.titleJournal of Endocrinology
dc.language.isoeng
dc.page.final267
dc.page.initial259
dc.publisherBioScientifica
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu612.017
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco24 Ciencias de la Vida
dc.titleEffects of glucose and insulin on glucokinase activity in rat hypothalamus
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number193
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery7e56a4f1-b1ee-4225-a0f8-6cfd1d9b9c85
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