Endogenous Hydrogen Sulfide has a Powerful Role in Inhibitory Neurotransmission to the Pig Bladder Neck

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dc.contributor.authorLeite Fernandes, Vitor Samuel
dc.contributor.authorRibeiro, Ana S.F.
dc.contributor.authorMartínez, María Pilar
dc.contributor.authorOrensanz, Luis M.
dc.contributor.authorBarahona Gomáriz, María Victoria
dc.contributor.authorMartínez-Sáenz, Ana
dc.contributor.authorRecio Visedo, María Paz
dc.contributor.authorBenedito Castellote, Sara
dc.contributor.authorBustamante, Salvador
dc.contributor.authorCarballido, Joaquín
dc.contributor.authorGarcía Sacristán, Albino
dc.contributor.authorPrieto Ocejo, Dolores
dc.contributor.authorHernández Rodríguez, Medardo Vicente
dc.date.accessioned2024-02-08T11:07:52Z
dc.date.available2024-02-08T11:07:52Z
dc.date.issued2013-04-01
dc.description.abstractPurpose: We investigated the possible involvement of H2S in nitric oxide independent inhibitory neurotransmission to the pig bladder neck. Materials and methods: We used immunohistochemistry to determine the expression of the H2S synthesis enzymes cystathionine γ-lyase and cystathionine β-synthase. We also used electrical field stimulation and myographs for isometric force recordings to study relaxation in response to endogenously released or exogenously applied H2S in urothelium denuded, phenylephrine precontracted bladder neck strips under noradrenergic, noncholinergic, nonnitrergic conditions. Results: Cystathionine γ-lyase and cystathionine β-synthase expression was observed in nerve fibers in the smooth muscle layer. Cystathionine γ-lyase and cystathionine β-synthase immunoreactive fibers were also identified around the small arteries supplying the bladder neck. Electrical field stimulation (2 to 16 Hz) evoked frequency dependent relaxation, which was decreased by DL-propargylglycine and abolished by tetrodotoxin (blockers of cystathionine γ-lyase and neuronal voltage gated Na(+) channels, respectively). The cystathionine β-synthase inhibitor O-(carboxymethyl)hydroxylamine did not change nerve mediated responses. The H2S donor GYY4137 (0.1 nM to 10 μM) induced potent, concentration dependent relaxation, which was not modified by neuronal voltage gated Na(+) channels, or cystathionine γ-lyase or cystathionine β-synthase blockade. Conclusions: Results suggest that endogenous H2S synthesized by cystathionine γ-lyase and released from intramural nerves acts as a powerful signaling molecule in nitric oxide independent inhibitory transmission to the pig bladder neck.
dc.description.departmentDepto. de Fisiología
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.sponsorshipFundação para a Ciência e Tecnologia, Ministério da Educação e Ciência (Portugal)
dc.description.statuspub
dc.identifier.doi10.1016/j.juro.2012.10.006
dc.identifier.issn0022-5347
dc.identifier.issn1527-3792
dc.identifier.officialurlhttps://doi.org/10.1016/j.juro.2012.10.006
dc.identifier.urihttps://hdl.handle.net/20.500.14352/100326
dc.issue.number4
dc.journal.titleJournal of Urology
dc.language.isoeng
dc.page.final1573
dc.page.initial1567
dc.publisherBaltimore : Lippincott Williams & Wilkins
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//PS09%2F00044/ES/MECANISMOS INVOLUCRADOS EN LA RELAJACION INDEPENDIENTE DE OXIDO NITRICO EN EL CUELLO DE LA VEJIGA URINARIA: IMPLICACION TERAPEUTICA EN LA INCONTINENCIA URINARIA PRODUCIDA POR DEFICIENCIA ESFINTERICA INTRINSECA/
dc.relation.projectIDinfo:eu-repo/grantAgreement/SFRH/ BD/68460/2010
dc.rights.accessRightsrestricted access
dc.subject.cdu591.1
dc.subject.cdu612.019
dc.subject.keywordUrinary bladder
dc.subject.keywordHydrogen sulfide
dc.subject.keywordSynaptic transmission
dc.subject.keywordElectric stimulation
dc.subject.keywordCystathionine gamma-lyase
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco2411 Fisiología Humana
dc.titleEndogenous Hydrogen Sulfide has a Powerful Role in Inhibitory Neurotransmission to the Pig Bladder Neck
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number189
dspace.entity.typePublication
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