Identification of the intermediate host of Gongylonema sp., the etiological agent of the necrotic oropharyngeal disease of the Scops owl (Otus scops)

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dc.contributor.authorLopes, Fatima
dc.contributor.authorEsperón, Fernando
dc.contributor.authorBravo-Barriga, D.
dc.contributor.authorFrontera, E.
dc.contributor.authorCabrero Sañudo, Francisco José
dc.contributor.authorGil Tapetado, Diego
dc.contributor.authorOrejas, P.
dc.contributor.authorAlonso, R.
dc.date.accessioned2023-06-16T14:24:46Z
dc.date.available2023-06-16T14:24:46Z
dc.date.issued2021-08-28
dc.description.abstractSince 1997, fledgling Scops owls (Chordata: Strigidae) have been brought to the Brinzal Owl Rescue Centre (Madrid, Spain) with severe lesions in their oral cavities. Lesions consist of the presence of proliferative necrotic material in the oral cavity resulting in white plaques, which can lead to death by starvation. This disease has been detected in owls only within the limits of the city of Madrid. The etiologic agent has been identified as Gongylonema sp. (Nematoda: Spirurida), a nematode genus that includes a coprophagous arthropod as intermediate host in its cycle. The aim of this study was to identify the intermediate host of the parasite. Our work was structured in four component phases: i) Diet study of newborn chicks; ii) trapping arthropods that could be intermediate hosts; iii) molecular detection of the parasite in the selected arthropods: and iv) molecular characterization of the detected parasites by amplifying the cox1 gene. Four male owls were radio-tagged in order to locate their nests and a camera trap was placed to identify the prey brought to the owlets. Secondly, the arthropods present in the hunting areas of the owls were sampled, identified and analyzed by real time PCR (rtPCR). Only oriental cockroaches, B. orientalis (Arthropoda: Blattodea), were positive by rtPCR detection of Gongylonema sp. (66.7%). The nematodes obtained from cockroaches had a 99.8% identity of the cox1 gene with the Gongylonema sp. isolated for the first time in a Scops owl. Furthermore, these sequences only showed an <89% identity with all the other Gongylonema sequences available in the GenBank database. We conclude that the oriental cockroach should be considered as an intermediate host of the etiologic agent of NOD.
dc.description.departmentDepto. de Biodiversidad, Ecología y Evolución
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMorris Animal Foundation
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/73504
dc.identifier.doi10.1016/j.parint.2021.102443
dc.identifier.issn1383-5769
dc.identifier.officialurlhttps://doi.org/10.1016/j.parint.2021.102443
dc.identifier.urihttps://hdl.handle.net/20.500.14352/4969
dc.journal.titleParasitology International
dc.language.isoeng
dc.page.final5
dc.page.initial1
dc.publisherElsevier
dc.relation.projectID(D14ZO-834)
dc.rights.accessRightsrestricted access
dc.subject.cdu598.279.42:591.2
dc.subject.keywordOriental cockroaches
dc.subject.keywordGongylonema sp.
dc.subject.keywordIntermediate host
dc.subject.keywordNecrotic oropharyngeal disease
dc.subject.keywordScops owl
dc.subject.ucmPatología veterinaria
dc.subject.ucmAves
dc.subject.unesco3109.07 Patología
dc.subject.unesco2401.20 Ornitología
dc.titleIdentification of the intermediate host of Gongylonema sp., the etiological agent of the necrotic oropharyngeal disease of the Scops owl (Otus scops)
dc.typejournal article
dc.volume.number86
dspace.entity.typePublication
relation.isAuthorOfPublication7ef7507d-8ea9-4389-82fc-b96803a0c1d4
relation.isAuthorOfPublication1edcbdf4-75d2-4bbe-b11b-4d15751d2988
relation.isAuthorOfPublication.latestForDiscovery7ef7507d-8ea9-4389-82fc-b96803a0c1d4
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