Aviso: para depositar documentos, por favor, inicia sesión e identifícate con tu cuenta de correo institucional de la UCM con el botón MI CUENTA UCM. No emplees la opción AUTENTICACIÓN CON CONTRASEÑA
 

Factor VIII mRNA expression from a BAC carrying the intact locus made by homologous recombination

Simple item page
dc.contributor.authorPérez Luz, Sara
dc.contributor.authorAbdulrazzak, Hassan
dc.contributor.authorGrillot-Courvalin, Catherine
dc.contributor.authorHuxley, Clare
dc.date.accessioned2024-01-15T10:21:04Z
dc.date.available2024-01-15T10:21:04Z
dc.date.issued2007
dc.description.abstractHemophilia A is caused by mutations in the gene encoding factor VIII (F8) and is an important target for gene therapy. The F8 gene contains 26 exons spread over ∼186 kb and no work using the intact genomic locus has been carried out. We have constructed a 250-kb BAC carrying all 26 exons, the introns, and more than 40 kb of upstream and 20 kb of downstream DNA. This F8 BAC was further retrofitted with either the oriP/EBNA-1 elements from Epstein–Barr virus, which allow episomal maintenance in mammalian cells, or alphoid DNA, which allows human artificial chromosome formation in some human cell lines. Lipofection of the oriP/EBNA-1-containing version into mouse Hepa1-6 cells resulted in expression of F8 mRNA spanning the F8 gene. The >300-kb BAC carrying alphoid DNA was successfully delivered to 293A and HT1080 cells using bacterial delivery, resulting in greater than endogenous levels of F8 mRNA expression.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Educación y Ciencia (España)
dc.description.sponsorshipMedical Research Council (United Kingdom)
dc.description.sponsorshipEuropean Commission
dc.description.statuspub
dc.identifier.citationPérez-Luz, Sara, et al. «Factor VIII mRNA Expression from a BAC Carrying the Intact Locus Made by Homologous Recombination». Genomics, vol. 90, n.o 5, noviembre de 2007, pp. 610-19. https://doi.org/10.1016/j.ygeno.2007.07.005.
dc.identifier.doi10.1016/j.ygeno.2007.07.005
dc.identifier.issn0888-7543
dc.identifier.officialurlhttps://doi.org/10.1016/j.ygeno.2007.07.005
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93001
dc.issue.number5
dc.language.isoeng
dc.page.final619
dc.page.initial610
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.cdu577
dc.subject.keywordHemophilia A
dc.subject.keywordFactor VIII
dc.subject.keywordF8
dc.subject.keywordGene therapy
dc.subject.keywordBacterial artificial chromosome
dc.subject.keywordBAC
dc.subject.keywordBacterial delivery
dc.subject.keywordOriP/EBNA-1
dc.subject.keywordAlphoid DNA
dc.subject.ucmBiología
dc.subject.ucmVeterinaria
dc.subject.unesco2415 Biología Molecular
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleFactor VIII mRNA expression from a BAC carrying the intact locus made by homologous recombination
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number90
dspace.entity.typePublication
relation.isAuthorOfPublication45168e0c-c225-4385-9386-0e750ca8ee09
relation.isAuthorOfPublication.latestForDiscovery45168e0c-c225-4385-9386-0e750ca8ee09

Download

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
FVIII.pdf
Size:
1.13 MB
Format:
Adobe Portable Document Format
Download

Collections

Artículos