Antibacterial effects of polymeric PolymP-n Active nanoparticles. An in vitro biofilm study

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dc.contributor.authorSánchez Beltrán, María Del Carmen
dc.contributor.authorToledano-Osorio, Manuel
dc.contributor.authorBueno, Jaime
dc.contributor.authorFiguero Ruiz, Elena
dc.contributor.authorToledano, Manuel
dc.contributor.authorMedina-Castillo, A.L.
dc.contributor.authorOsorio, Raquel
dc.contributor.authorHerrera González, David
dc.contributor.authorSanz Alonso, Mariano
dc.date.accessioned2024-01-24T11:18:25Z
dc.date.available2024-01-24T11:18:25Z
dc.date.issued2019
dc.description.abstractObjective: to study the antibacterial effect of polymeric PolymP-n Active nanoparticles using an in vitro subgingival biofilm model. Methods: Hydroxyapatite discs coated with five modalities of nanoparticles (NPs): NPs, NPs doped with zinc, calcium, silver and doxycycline, PBS as control, and Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans were studied in a static in vitro biofilm model (12, 24, 48, and 72h). Nano-roughness of the different disc surfaces (SRa, in nm) and morphological characteristic of the biofilms (thickness (μm) and bacterial viability) were studied by different microscopy modalities. Quantitative Polymerase Chain Reaction was used to assess the effect of the nanoparticles on the bacterial load (colony forming unit per milliliter) (CFUmL-1). Analysis of variance and post-hoc testing with T3 Dunnett́s, and Student Newman Keuls correction was used. Results were considered statistically significant at p<0.05. Results: Surfaces containing the different nanoparticles showed significant increments in roughness when compared to controls (p<0.05). A similar biofilm formation and dynamics was observed, although reductions in bacterial viability were detected in biofilms in contact with the different nanoparticles, more pronounced with silver and doxycycline NPs. Doxycycline-NPs biofilms resulted in unstructured biofilm formation and significantly lower number of the six species when compared with the other nanoparticles specimens and controls (p<0.001 in all cases). Significance: Polymeric PolymP-n Active nanoparticles when combined with silver and doxycycline showed a significant antibacterial effect when tested in an in vitro subgingival biofilm model.
dc.description.departmentDepto. de Especialidades Clínicas Odontológicas
dc.description.facultyFac. de Odontología
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationSánchez MC, Toledano-Osorio M, Bueno J, Figuero E, Toledano M, Medina-Castillo AL, Osorio R, Herrera D, Sanz M. Antibacterial effects of polymeric PolymP-n Active nanoparticles. An in vitro biofilm study. Dent Mater. 2019 Jan;35(1):156-168
dc.identifier.doi10.1016/j.dental.2018.11.015
dc.identifier.essn1879-0097
dc.identifier.issn0109-5641
dc.identifier.officialurlhttps://www.doi.org/10.1016/j.dental.2018.11.015
dc.identifier.pmid30502966
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95044
dc.issue.number1
dc.journal.titleDental Materials
dc.language.isoeng
dc.page.final168
dc.page.initial156
dc.publisherElsevier
dc.rights.accessRightsrestricted access
dc.subject.cdu616.314
dc.subject.cdu616.314.17-008.1
dc.subject.cdu579.61
dc.subject.keywordCalcium
dc.subject.keywordDoxycycline
dc.subject.keywordNanoparticles
dc.subject.keywordOral biofilm
dc.subject.keywordSilver
dc.subject.keywordZinc
dc.subject.ucmOdontología (Odontología)
dc.subject.ucmPeriodoncia
dc.subject.ucmMicrobiología médica
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco3213.13 Ortodoncia-Estomatología
dc.subject.unesco3201.03 Microbiología Clínica
dc.titleAntibacterial effects of polymeric PolymP-n Active nanoparticles. An in vitro biofilm study
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number35
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery27fce85c-05b6-4420-b0a4-22ef8510f4e2
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