Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria

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dc.contributor.authorFernández Veiga, Leire
dc.contributor.authorFuertes, Miguel
dc.contributor.authorGeijo, María V.
dc.contributor.authorPérez de Val, Bernat
dc.contributor.authorVidal, Enric
dc.contributor.authorMichelet, Lorraine
dc.contributor.authorBoschiroli, María Laura
dc.contributor.authorGómez Buendía, Alberto
dc.contributor.authorBezos Garrido, Javier
dc.contributor.authorJones, Gareth J.
dc.contributor.authorVordermeier, Martin
dc.contributor.authorJuste, Ramón A.
dc.contributor.authorGarrido, Joseba M.
dc.contributor.authorSevilla, Iker A.
dc.date.accessioned2024-08-22T06:26:27Z
dc.date.available2024-08-22T06:26:27Z
dc.date.issued2023-02-20
dc.descriptionAuthor contributions J.M.G. and I.A.S. conceived and planned the study and obtained the funding. B.P.V., L.M., M.L.B., J.B., G.J.J., H.M.V., J.M.G. and I.A.S. contributed to choosing isolates and/or deciding skin test protocols and antigens. B.P.V., L.M. and M.L.B. provided isolates. G.J.J. and H.M.V. provided antigens. L.F.V., M.F., M.V.G., J.M.G. and I.A.S. performed the experiments and laboratory analysis and compiled the data. R.A.J., J.M.G. and I.A.S. analyzed and interpreted the results. R.A.J., J.M.G. and I.A.S. wrote the original draf of the manuscript. All authors reviewed the original draft and contributed to writing and editing the final version of the manuscript. Additional information Correspondence and requests for materials should be addressed to I.A.S.
dc.description.abstractThe single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity.
dc.description.departmentDepto. de Sanidad Animal
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades
dc.description.sponsorshipINTERREG POCTEFA
dc.description.sponsorshipEuropean Regional Development Fund
dc.description.statuspub
dc.identifier.citationFernández-Veiga, L., Fuertes, M., Geijo, M.V. et al. Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria. Sci Rep 13, 2936 (2023). https://doi.org/10.1038/s41598-023-30147-4
dc.identifier.doi10.1038/s41598-023-30147-4
dc.identifier.issn2045-2322
dc.identifier.officialurlhttps://doi.org/10.1038/s41598-023-30147-4
dc.identifier.pmid36806813
dc.identifier.relatedurlhttps://www.nature.com/articles/s41598-023-30147-4
dc.identifier.urihttps://hdl.handle.net/20.500.14352/107603
dc.issue.number2936
dc.journal.titleScientific reports
dc.language.isoeng
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105155RB-C33/ES/DEVELOPMENT OF INACTIVATED VACCINE PROTOTYPES FOR THE CONTROL OF TB IN DOMESTIC ANIMALS AND ASSESSMENT OF THE INTEREFERENCE CAUSED BY NON TUBERCULOUS ORGANISMS ON ITS DIAGNOSI/
dc.relation.projectIDEFA357/19
dc.relation.projectIDMCIN/ AEI/10.13039/501100011033
dc.relation.projectIDPID2019-105155RB-C33
dc.relation.projectIDEFA357/19 INNOTUB
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.cdu636.09
dc.subject.keywordInmunology
dc.subject.keywordInfectious-disease diagnostics
dc.subject.keywordMicrobiology
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleDifferences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
dc.typejournal article
dc.type.hasVersionAM
dc.volume.number13
dspace.entity.typePublication
relation.isAuthorOfPublication2e80b9e6-3809-4756-9ef8-ebc9ce005eb8
relation.isAuthorOfPublication.latestForDiscovery2e80b9e6-3809-4756-9ef8-ebc9ce005eb8

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