Stress-Induced Microspore Embryogenesis Requires Endogenous Auxin Synthesis and Polar Transport in Barley.

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dc.contributor.authorPérez-Pérez, Yolanda
dc.contributor.authorEl-Tantawy, Ahmed
dc.contributor.authorSolís González, María Teresa
dc.contributor.authorSolís, María Teresa
dc.contributor.authorRisueño, Maía del Carmen
dc.contributor.authorTestillano, Pilar
dc.date.accessioned2024-02-01T15:36:18Z
dc.date.available2024-02-01T15:36:18Z
dc.date.issued2019
dc.description.abstractStress-induced microspore embryogenesis is a model in vitro system of cell reprogramming, totipotency acquisition, and embryo development. After induction, responsive microspores abandon their developmental program to follow an embryogenic pathway, leading to in vitro embryo formation. This process is widely used to produce doubled-haploid lines, essential players to create new materials in modern breeding programs, particularly in cereals, although its efficiency is still low in many crop species, because the regulating mechanisms are still elusive. Stress signaling and endogenous hormones, mainly auxin, have been proposed as determinant factors of microspore embryogenesis induction in some eudicot species; however, much less information is available in monocot plants. In this study, we have analyzed the dynamics and possible role of endogenous auxin during stress-induced microspore embryogenesis in the monocot Hordeum vulgare, barley. The results showed auxin accumulation in early proembryo cells, from embryogenesis initiation and a further increase with embryo development and differentiation, correlating with the induction and expression pattern of the auxin biosynthesis gene HvTAR2-like. Pharmacological treatments with kynurenine, inhibitor of auxin biosynthesis, and α-(p-chlorophenoxy)-isobutyric acid (PCIB), auxin antagonist, impaired embryogenesis initiation and development, indicating that de novo auxin synthesis and its activity were required for the process. Efflux carrier gene HvPIN1-like was also induced with embryogenesis initiation and progression; auxin transport inhibition by N-1-naphthylphthalamic acid significantly reduced embryo development at early and advanced stages. The results indicate activation of auxin biosynthesis with microspore embryogenesis initiation and progression, in parallel with the activation of polar auxin transport, and reveal a central role of auxin in the process in a monocot species. The findings give new insights into the complex regulation of stress-induced microspore embryogenesis, particularly in monocot plants for which information is still scarce, and suggest that manipulation of endogenous auxin content could be a target to improve in vitro embryo production.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas
dc.description.statuspub
dc.identifier.citationPérez-Pérez Y, El-Tantawy AA, Solís MT, Risueño MC, Testillano PS. Stress-Induced Microspore Embryogenesis Requires Endogenous Auxin Synthesis and Polar Transport in Barley. Front Plant Sci. 2019 Sep 27;10:1200. doi: 10.3389/fpls.2019.01200. PMID: 31611902; PMCID: PMC6776631.
dc.identifier.doi10.3389/FPLS.2019.01200
dc.identifier.essn1664-462X
dc.identifier.officialurlhttps://doi.org/10.3389/fpls.2019.01200/full
dc.identifier.pmid31611902
dc.identifier.urihttps://hdl.handle.net/20.500.14352/97864
dc.journal.titleFrontiers in Plant Science
dc.language.isoeng
dc.publisherFrontiers
dc.relation.projectIDAGL2017- 82447-R
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//AGL2014-52028-R/ES/FACTORES DETERMINANTES DE LA REPROGRAMACION A EMBRIOGENESIS DE LA MICROSPORA INDUCIDA POR ESTRES PARA MEJORA DE PLANTAS CULTIVADAS/
dc.rightsAttribution 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordHordeum vulgare
dc.subject.keywordN-1-naphthylphthalamic acid
dc.subject.keywordAuxin
dc.subject.keywordBarley
dc.subject.keywordCell reprogramming
dc.subject.keywordMicrospore embryogenesis
dc.subject.keywordKynurenine
dc.subject.keywordα-(p-chlorophenoxy)-isobutyric acid
dc.subject.ucmCiencias
dc.subject.unesco31 Ciencias Agrarias
dc.titleStress-Induced Microspore Embryogenesis Requires Endogenous Auxin Synthesis and Polar Transport in Barley.
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublication3da5e099-3087-4d19-8ed0-60a12692e004
relation.isAuthorOfPublication.latestForDiscovery3da5e099-3087-4d19-8ed0-60a12692e004
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