Tonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat-inactivated Mycobacterium bovis

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dc.contributor.authorBeltrán-Beck, Beatriz
dc.contributor.authorBoadella, Mariana
dc.contributor.authorCasal, Carmen
dc.contributor.authorBezos Garrido, Javier
dc.contributor.authorMazariegos Martínez-Peñalver, María
dc.contributor.authorMartín, MariPaz
dc.contributor.authorGalindo, Ruth C
dc.contributor.authorPérez de la Lastra, José M
dc.contributor.authorVillar, Margarita
dc.contributor.authorGarrido, Joseba M
dc.contributor.authorSevilla, Iker A
dc.contributor.authorAsensio, Fernando
dc.contributor.authorSicilia, Javier
dc.contributor.authorLyashchenko, Konstantin P
dc.contributor.authorJuste, Ramón A
dc.contributor.authorde la Fuente, José
dc.contributor.authorGortázar, Christian
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.contributor.authorRomero Martínez, Beatriz
dc.date.accessioned2023-06-19T15:04:32Z
dc.date.available2023-06-19T15:04:32Z
dc.date.issued2014-08
dc.description.abstractMycobacterium bovis causes animal tuberculosis (TB) in cattle, humans, and other mammalian species, including pigs. The goal of this study was to experimentally assess the responses of pigs with and without a history of tonsillectomy to oral vaccination with heat-inactivated M. bovis and challenge with a virulent M. bovis field strain, to compare pig and wild boar responses using the same vaccination model as previously used in the Eurasian wild boar (Sus scrofa), to evaluate the use of several enzyme-linked immunosorbent assays (ELISAs) and lateral flow tests for in vivo TB diagnosis in pigs, and to verify if these tests are influenced by oral vaccination with inactivated M. bovis. At necropsy, the lesion and culture scores were 20% to 43% higher in the controls than those in the vaccinated pigs. Massive M. bovis growth from thoracic tissue samples was observed in 4 out of 9 controls but in none of the 10 vaccinated pigs. No effect of the presence or absence of tonsils was observed on these scores, suggesting that tonsils are not involved in the protective response to this vaccine in pigs. The serum antibody levels increased significantly only after challenge. At necropsy, the estimated sensitivities of the ELISAs and dual path platform (DPP) assays ranged from 89% to 94%. In the oral mucosa, no differences in gene expression were observed in the control group between the pigs with and without tonsils. In the vaccinated group, the mRNA levels for chemokine (C-C motif) receptor 7 (CCR7), interferon beta (IFN-β), and methylmalonyl coenzyme A mutase (MUT) were higher in pigs with tonsils. Complement component 3 mRNA levels in peripheral blood mononuclear cells (PBMC) increased with vaccination and decreased after M. bovis challenge. This information is relevant for pig production in regions that are endemic for M. bovis and for TB vaccine research.
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. FP7
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39634
dc.identifier.doi10.1128/CVI.00221-14
dc.identifier.issn1556-679X
dc.identifier.officialurlhttp://cvi.asm.org/content/21/8/1128.full
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35275
dc.issue.number8
dc.journal.titleClinical and vaccine immunology
dc.language.isoeng
dc.page.final36
dc.page.initial1128
dc.publisherAmerican Society for Microbiology
dc.relation.projectIDWILDTBVAC (613779)
dc.relation.projectIDAGL2011-30041
dc.rights.accessRightsrestricted access
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleTonsils of the soft palate do not mediate the response of pigs to oral vaccination with heat-inactivated Mycobacterium bovis
dc.typejournal article
dc.volume.number21
dspace.entity.typePublication
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relation.isAuthorOfPublicationb1500641-3851-4564-8164-2721e44577e9
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relation.isAuthorOfPublication.latestForDiscovery2e80b9e6-3809-4756-9ef8-ebc9ce005eb8
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