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Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1

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dc.contributor.authorArrieta, Ignacio
dc.contributor.authorBarrio, Manuel Del
dc.contributor.authorVidarte, Luis
dc.contributor.authorPozo, Victoria del
dc.contributor.authorPastor Vargas, Carlos
dc.contributor.authorGonzález Cabrero, Jesús
dc.contributor.authorCárdaba, Blanca
dc.contributor.authorRojo, Marta
dc.contributor.authorMı́nguez, Ascensión
dc.contributor.authorCortegano Jimeno, María Isabel
dc.date.accessioned2024-01-29T16:10:02Z
dc.date.available2024-01-29T16:10:02Z
dc.date.issued2000
dc.description.abstractIngestion of the parasitic nematode Anisakis simplex in undercooked fish can cause severe allergic reactions in some individuals. Using pooled human sera from sensitized patients we have probed an expression library for A. simplex antigens. One positive clone was found to encode a full length 21 kDa protein with strong homology to nematode troponins. The recombinant protein was expressed as a GST-fusion protein and found by immunoblot analysis to react with sera from 20% of allergic patients. The presence of functional EF-hand Ca(2+) binding motifs was demonstrated by gel-shift analysis.en
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipSociedad Española de Alergia e Inmunologı́a Clı́nica
dc.description.statuspub
dc.identifier.citationArrieta I, del Barrio M, Vidarte L, del Pozo V, Pastor C, Gonzalez-Cabrero J, Cárdaba B, Rojo M, Mínguez A, Cortegano I, Gallardo S, Aceituno E, Palomino P, Vivanco F, Lahoz C. Molecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1. Mol Biochem Parasitol. 2000 Apr 15;107(2):263-8. doi: 10.1016/s0166-6851(00)00192-4. PMID: 10779602.
dc.identifier.doi10.1016/s0166-6851(00)00192-4
dc.identifier.issn0166-6851
dc.identifier.officialurlhttps://doi.org/10.1016/s0166-6851(00)00192-4
dc.identifier.pmid10779602
dc.identifier.urihttps://hdl.handle.net/20.500.14352/96205
dc.issue.number2
dc.journal.titleMolecular and Biochemical Parasitology
dc.language.isoeng
dc.page.final268
dc.page.initial263
dc.publisherElsevier
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu577.1
dc.subject.keywordHelminth Parasite
dc.subject.keywordHypersensitivity
dc.subject.keywordAnisakis simplex
dc.subject.ucmCiencias Biomédicas
dc.subject.unesco3207.01 Alergias
dc.titleMolecular cloning and characterization of an IgE-reactive protein from Anisakis simplex: Ani s 1en
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number107
dspace.entity.typePublication
relation.isAuthorOfPublication25af78c7-0077-4891-a14e-bcd8e51fe408
relation.isAuthorOfPublication9717f9eb-5b18-4825-8850-3f5644af61c5
relation.isAuthorOfPublication.latestForDiscovery25af78c7-0077-4891-a14e-bcd8e51fe408

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