A down-scaled fluorimetric determination of the solubility properties of drugs to minimize waste generation

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dc.contributor.authorGonzález Ruiz, Víctor
dc.contributor.authorOlives Barba, Ana Isabel
dc.contributor.authorMartín Carmona, María Antonia
dc.date.accessioned2024-02-07T10:38:14Z
dc.date.available2024-02-07T10:38:14Z
dc.date.issued2013
dc.description.abstractA miniaturized fluorescence assay on multi-well plates has been developed to study the solubility enhancement effect of (2-hydroxypropyl)-β-cyclodextrin on three anti-tumor alkaloids. The measurement of the fluorescence emission on a multi-well plate format has been proved to be a rapid and efficient technique to evaluate the solubility of pharmaceutical formulations of new drugs that help save time, reagents and wastes in the search for greener analytical strategies. The proposed methodology was compared with a reference HPLC solubility study and was employed to examine the enhancement of the solubility of camptothecin, luotonin A, and a synthetic derivative of the latter in the presence of (2-hydroxypropyl)-β-cyclodextrin. Considerable reductions in the time of analysis (almost 50 times faster) and the volume of organic solvents employed (close to 25 times less acetonitrile needed) were achieved. The nature of the inclusion complexes was investigated by analysis of the phase-solubility diagrams obtained by the newly developed method and was complemented with spectrofluorimetry and ESI-MS experiments. The concentrations of solubilised compounds found by both methodologies were in good agreement (R2 > 0.98). The analytical figures of merit of both methodologies were compared and the adequacy of the proposed method for the development of drug solubilisation studies was discussed.s.eng
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (España)
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.identifier.citationGonzález-Ruiz V, Olives AI, Martín MA. A down-scaled fluorimetric determination of the solubility properties of drugs to minimize waste generation. Green Chem 2013;15:2558. https://doi.org/10.1039/c3gc40974k.
dc.identifier.doi10.1039/c3gc40974k
dc.identifier.essn1463-9270
dc.identifier.issn1463-9262
dc.identifier.officialurlhttps://doi.org/10.1039/c3gc40974k
dc.identifier.urihttps://hdl.handle.net/20.500.14352/99876
dc.journal.titleGreen Chemistry
dc.language.isoeng
dc.relation.projectIDinfo:eu-repo/grantAgreement/CTQ2009-11312/BQU
dc.relation.projectIDinfo:eu-repo/grantAgreement/GR35/10-A-920234
dc.rights.accessRightsrestricted access
dc.subject.cdu615:54
dc.subject.cdu615.31
dc.subject.ucmQuímica analítica (Química)
dc.subject.ucmQuímica farmaceútica
dc.subject.unesco2301 Química Analítica
dc.titleA down-scaled fluorimetric determination of the solubility properties of drugs to minimize waste generation
dc.typejournal article
dc.type.hasVersionAM
dspace.entity.typePublication
relation.isAuthorOfPublication109c167c-4d5d-41f6-be94-4ca6160136a5
relation.isAuthorOfPublication711c3146-caca-4061-9626-60a946a50f20
relation.isAuthorOfPublication.latestForDiscovery109c167c-4d5d-41f6-be94-4ca6160136a5
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