Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii

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dc.contributor.authorMorales Menchén, Ana
dc.contributor.authorNavarro García, Federico
dc.contributor.authorGuirao Abad, José P.
dc.contributor.authorRomán González, Elvira
dc.contributor.authorPrieto Prieto, Antonio Daniel
dc.contributor.authorComan, Ioana V
dc.contributor.authorPla Alonso, Jesús
dc.contributor.authorAlonso Monge, Rebeca María Del Mar
dc.date.accessioned2023-06-17T13:23:57Z
dc.date.available2023-06-17T13:23:57Z
dc.date.issued2018-05-03
dc.description.abstractCertain yeasts secrete peptides known as killer toxins or mycocins with a deleterious effect on sensitive yeasts or filamentous fungi, a common phenomenon in environmental species. In a recent work, different () strains isolated from a wide variety of cheeses were identified as producing killer toxins active against and . We have analyzed the killer activity of these toxins in mutants defective in MAPK signaling pathways and found that the lack of the MAPK Hog1 (but not Cek1 or Mkc1) renders cells hypersensitive to mycocins while mutants lacking other upstream elements of the pathway behave as the wild type strain. Point mutations in the phosphorylation site (T174A-176F) or in the kinase domain (K52R) of gene showed that both activities were relevant for the survival of to killer toxins. Moreover, Hog1 phosphorylation was also required to sense and adapt to osmotic and oxidative stress while the kinase activity was somehow dispensable. Although the addition of supernatant from the killer toxin- producing 242 strain (-242) induced a slight intracellular increase in Reactive Oxygen Species (ROS), overexpression of cytosolic catalase did not protect against this mycocin. This supernatant induced an increase in intracellular glycerol concentration suggesting that this toxin triggers an osmotic stress. We also provide evidence of a correlation between sensitivity to -242 killer toxin and resistance to Congo red, suggesting cell wall specific alterations in sensitive strains.en
dc.description.departmentDepto. de Microbiología y Parasitología
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Comercio y Empresa (España)
dc.description.sponsorshipCoordination of European Funding for Infectious Diseases Research
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/55650
dc.identifier.citationMorales Menchén, A., Navarro García, F., Guirao Abad, J. P. et al. «Non-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hansenii». Frontiers in Cellular and Infection Microbiology, vol. 8, mayo de 2018, p. 135. DOI.org (Crossref), https://doi.org/10.3389/fcimb.2018.00135.
dc.identifier.doi10.3389/fcimb.2018.00135
dc.identifier.issn2235-2988
dc.identifier.officialurlhttps//doi.org/10.3389/fcimb.2018.00135
dc.identifier.urihttps://hdl.handle.net/20.500.14352/13366
dc.journal.titleFrontiers in cellular and infection microbiology
dc.language.isoeng
dc.page.initial135
dc.relation.projectIDBIO2015-64777-P
dc.relation.projectIDPCIN-2014-052
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu579
dc.subject.keywordKiller toxin
dc.subject.keywordOsmotic stress
dc.subject.keywordDebaryomyces hansenii
dc.subject.keywordMAPK
dc.subject.keywordCandida albicans
dc.subject.keywordHOG pathway
dc.subject.ucmMicrobiología (Farmacia)
dc.subject.unesco3302.03 Microbiología Industrial
dc.titleNon-canonical Activities of Hog1 Control Sensitivity of Candida albicans to Killer Toxins From Debaryomyces hanseniien
dc.typejournal article
dc.volume.number8
dspace.entity.typePublication
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